en
Scientific article
Open access
English

Cx26 regulates proliferation of repairing basal airway epithelial cells

Publication date2014
Abstract

The recovery of an intact epithelium following injury is critical for restoration of lung homeostasis, a process that may be altered in cystic fibrosis (CF). In response to injury, progenitor cells in the undamaged areas migrate, proliferate and re-differentiate to regenerate an intact airway epithelium. The mechanisms regulating this regenerative response are, however, not well understood. In a model of circular wound injury of well-differentiated human airway epithelial cell (HAEC) cultures, we identified the gap junction protein Cx26 as an important regulator of cell proliferation. We report that induction of Cx26 in repairing HAECs is associated with cell proliferation. We also show that Cx26 is expressed in a population of CK14-positive basal-like cells. Cx26 silencing in immortalized cell lines using siRNA and in primary HAECs using lentiviral-transduced shRNA enhanced Ki67-labeling index and Ki67 mRNA, indicating that Cx26 acts a negative regulator of HAEC proliferation. Cx26 silencing also markedly decreased the transcription of KLF4 in immortalized HAECs. We further show that CF HAECs exhibited deregulated expression of KLF4, Ki67 and Cx26 as well enhanced rate of wound closure in the early response to injury. These results point to an altered repair process of CF HAECs characterized by rapid but desynchronized initiation of HAEC activation and proliferation. This article is part of a Directed Issue entitled: Cystic fibrosis: From o-mics to cell biology, physiology, and therapeutic advances.

Citation (ISO format)
CRESPIN, Sophie et al. Cx26 regulates proliferation of repairing basal airway epithelial cells. In: International journal of biochemistry & cell biology, 2014, vol. 52, p. 152–160. doi: 10.1016/j.biocel.2014.02.010
Main files (1)
Article (Accepted version)
accessLevelPublic
Identifiers
ISSN of the journal1357-2725
789views
511downloads

Technical informations

Creation05/16/2014 8:36:00 AM
First validation05/16/2014 8:36:00 AM
Update time03/14/2023 9:15:41 PM
Status update03/14/2023 9:15:41 PM
Last indexation05/02/2024 3:14:28 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack