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Title

Anti-HLA antibody repertoire after IVIg infusion in highly sensitized patients waiting for kidney transplantation

Authors
Andresen, Irmgard
Binet, Isabelle
Published in Swiss Medical Weekly. 2006, vol. 136, no. 43-44, p. 696-702
Abstract Polyclonal intravenous immunoglobulin (IVIg) treatment reduces crossmatch positivity and increases rates of transplantation in highly sensitised patients (HS). We quantified the panel reactive antibody (PRA) by microlymphocytotoxicity (MLCC), and we analysed anti-HLA class I and class II IgG specific antibody repertoire by Luminex before and after IVIg infusion alone in HS patients awaiting kidney transplantation. Five patients received three monthly infusions of 1 g/kg of IVIg. Serum samples collected pre and post IVIg treatment were submitted for PRA analysis by MLCC. Anti-class I and anti-class II antibody specificities were then tested by Luminex. We focused on the anti-HLA class I and class II antibodies directed against HLA expressed by a previous graft. We also analysed the anti-HLA antibody repertoire in three patients who had not received IVIg infusion. The PRA level determined by MLCC decreased significantly in one of the five patients, dropping from 40% to 17%. The Luminex assay showed fluctuations of the anti-HLA antibody levels over time, but no significant longterm modifications of the anti-HLA antibody repertoire were observed, even in the patient with a strong and prolonged reduction of the PRA determined by MLCC. Our results show that IVIg at 1 g/kg is not sufficient to reduce PRA and does not modify the repertoire of specific anti-HLA antibody determined by Luminex.
Keywords AdultAgedAntibodies/bloodCase-Control StudiesFemaleGraft Rejection/prevention & controlHLA Antigens/immunologyHumansImmunoglobulins, Intravenous/therapeutic useKidney Transplantation/immunologyMaleMiddle AgedPilot ProjectsTransplantation Conditioning/methods
Identifiers
PMID: 17183432
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Structures
Research groups Transplantation Immunologie et Osteoimmunologie (561)
Cultures cellulaires et transplantations (519)
Groupe Pierre-Yves Martin (néphrologie) (30)
Citation
(ISO format)
FERRARI-LACRAZ, Sylvie et al. Anti-HLA antibody repertoire after IVIg infusion in highly sensitized patients waiting for kidney transplantation. In: Swiss Medical Weekly, 2006, vol. 136, n° 43-44, p. 696-702. https://archive-ouverte.unige.ch/unige:36668

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Deposited on : 2014-05-16

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