UNIGE document Scientific Article
previous document  unige:36375  next document
add to browser collection
Title

Structural basis for polyadenosine-RNA binding by Nab2 Zn fingers and its function in mRNA nuclear export

Authors
Brockmann, Christoph
Soucek, Sharon
Kuhlmann, Sonja I.
Mills-Lujan, Katherine
Kelly, Seth M.
Yang, Ji-Chun
show hidden authors show all authors [1 - 11]
Published in Structure. 2012, vol. 20, no. 6, p. 1007-18
Abstract Polyadenylation regulation and efficient nuclear export of mature mRNPs both require the polyadenosine-RNA-binding protein, Nab2, which contains seven CCCH Zn fingers. We describe here the solution structure of fingers 5-7, which are necessary and sufficient for high-affinity polyadenosine-RNA binding, and identify key residues involved. These Zn fingers form a single structural unit. Structural coherence is lost in the RNA-binding compromised Nab2-C437S mutant, which also suppresses the rat8-2 allele of RNA helicase Dbp5. Structure-guided Nab2 variants indicate that dbp5(rat8-2) suppression is more closely linked to hyperadenylation and suppression of mutant alleles of the nuclear RNA export adaptor, Yra1, than to affinity for polyadenosine-RNA. These results indicate that, in addition to modulating polyA tail length, Nab2 has an unanticipated function associated with generating export-competent mRNPs, and that changes within fingers 5-7 lead to suboptimal assembly of mRNP export complexes that are more easily disassembled by Dbp5 upon reaching the cytoplasm.
Keywords Active Transport, Cell NucleusAdenosine/chemistryAmino Acid SequenceAmino Acid SubstitutionConserved SequenceModels, MolecularMolecular Sequence DataMutagenesis, Site-DirectedNuclear Magnetic Resonance, BiomolecularNucleocytoplasmic Transport Proteins/chemistry/genetics/metabolismPolymers/chemistryProtein BindingProtein Structure, TertiaryRNA TransportRNA, Messenger/chemistryRNA-Binding Proteins/chemistry/genetics/metabolismSaccharomyces cerevisiae/genetics/growth & developmentSaccharomyces cerevisiae Proteins/chemistry/genetics/metabolismSurface PropertiesThermodynamicsZinc Fingers
Identifiers
PMID: 22560733
Full text
Article (Published version) (1.6 MB) - public document Free access
Structures
Research group Groupe Stutz
Project FNS: 31003A_130292
Citation
(ISO format)
BROCKMANN, Christoph et al. Structural basis for polyadenosine-RNA binding by Nab2 Zn fingers and its function in mRNA nuclear export. In: Structure, 2012, vol. 20, n° 6, p. 1007-18. https://archive-ouverte.unige.ch/unige:36375

305 hits

166 downloads

Update

Deposited on : 2014-05-06

Export document
Format :
Citation style :