The molecular scanner: concept and developments

Binz, Pierre-Alain; Muller, Markus Johann; Hoogland, Christine; Zimmermann, Catherine; Pasquarello Mosimann, Carla; Corthals, Garry; Sanchez, Jean-Charles; Hochstrasser, Denis; Appel, Ron David

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Title		The molecular scanner: concept and developments
Authors		Binz, Pierre-Alain Muller, Markus Johann Hoogland, Christine Zimmermann, Catherine Pasquarello Mosimann, Carla Corthals, Garry Sanchez, Jean-Charles Hochstrasser, Denis Appel, Ron David show all authors [1 - 9]
Published in		Current Opinion in Biotechnology. 2004, vol. 15, no. 1, p. 17-23
Abstract		Approaches aimed at deciphering the proteome have illustrated the need for relatively complex and highly sensitive methodologies. The major elements of proteome analysis, such as powerful protein separation and enzymatic processing, mass spectrometry and dedicated bioinformatics have been assembled in the development of the molecular scanner. This highly flexible and data-rich approach has combined the power of electrophoretic protein separation, the simultaneous digestion and transfer of proteins through an enzymatic membrane, the immediate use of the MALDI mass spectrometer to scan a collecting membrane, and the development of dedicated bioinformatics tools to perform protein identification and molecular imaging of the proteome. Clinical applications of the molecular scanner have also started to be developed for disease diagnosis in biological material.
Keywords		Animals — Biotechnology/instrumentation/methods/trends — Electrophoresis, Gel, Two-Dimensional/instrumentation/methods — Equipment Design — Gene Expression Profiling/instrumentation/methods/trends — Humans — Proteins/analysis/chemistry — Proteomics/instrumentation/methods/trends — Sequence Analysis, Protein/instrumentation/methods/trends — Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/instrumentation/methods/trends — Systems Integration — User-Computer Interface
Identifiers		DOI: 10.1016/j.copbio.2003.12.003 PMID: 15102461
Full text		Article (Published version) (205 Kb) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique
Research group		Groupe de Protéomique biomédicale (635)
Citation (ISO format)		BINZ, Pierre-Alain et al. The molecular scanner: concept and developments. In: Current Opinion in Biotechnology, 2004, vol. 15, n° 1, p. 17-23. https://archive-ouverte.unige.ch/unige:36215