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Article scientifique
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Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors

Contributeurs/tricesFish, Richardorcid; Kruithof, Egbert
Publié dansBMC molecular biology, vol. 5, 9
Date de publication2004
Résumé

RNA interference (RNAi) can potently reduce target gene expression in mammalian cells and is in wide use for loss-of-function studies. Several recent reports have demonstrated that short double-stranded RNAs (dsRNAs), used to mediate RNAi, can also induce an interferon-based response resulting in changes in the expression of many interferon-responsive genes. Off-target gene silencing has also been described, bringing into question the validity of certain RNAi-based approaches for studying gene function. We have targeted the plasminogen activator inhibitor-2 (PAI-2 or SERPINB2) mRNA using lentiviral vectors for delivery of U6 promoter-driven PAI-2-targeted short hairpin RNA (shRNA) expression. PAI-2 is reported to have anti-apoptotic activity, thus reduction of endogenous expression may be expected to make cells more sensitive to programmed cell death.

Mots-clés
  • 2',5'-Oligoadenylate Synthetase/genetics
  • Biological Markers/metabolism
  • Cell Line
  • Cell Line, Tumor
  • Drug Delivery Systems/methods
  • Enzyme Induction/genetics
  • Gene Expression/genetics
  • Gene Silencing
  • Gene Transfer Techniques
  • Genetic Vectors/genetics
  • HeLa Cells/drug effects
  • Humans
  • Kidney/cytology/drug effects/embryology
  • Lentivirus/genetics/metabolism
  • Plasminogen Activator Inhibitor 2/biosynthesis/genetics/metabolism
  • RNA Stability
  • RNA, Antisense/administration & dosage/adverse effects/metabolism
  • RNA, Messenger/genetics/metabolism
  • RNA, Small Interfering/adverse effects/biosynthesis/metabolism
  • Transduction, Genetic/methods
Citation (format ISO)
FISH, Richard, KRUITHOF, Egbert. Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors. In: BMC molecular biology, 2004, vol. 5, p. 9. doi: 10.1186/1471-2199-5-9
Fichiers principaux (1)
Article (Published version)
accessLevelPublic
Identifiants
ISSN du journal1471-2199
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Création11/04/2014 14:24:00
Première validation11/04/2014 14:24:00
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