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Title

Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors
Authors
Fish, Richard
Kruithof, Egbert
Published in BMC Molecular Biology. 2004, vol. 5, p. 9
Abstract RNA interference (RNAi) can potently reduce target gene expression in mammalian cells and is in wide use for loss-of-function studies. Several recent reports have demonstrated that short double-stranded RNAs (dsRNAs), used to mediate RNAi, can also induce an interferon-based response resulting in changes in the expression of many interferon-responsive genes. Off-target gene silencing has also been described, bringing into question the validity of certain RNAi-based approaches for studying gene function. We have targeted the plasminogen activator inhibitor-2 (PAI-2 or SERPINB2) mRNA using lentiviral vectors for delivery of U6 promoter-driven PAI-2-targeted short hairpin RNA (shRNA) expression. PAI-2 is reported to have anti-apoptotic activity, thus reduction of endogenous expression may be expected to make cells more sensitive to programmed cell death.
Keywords 2',5'-Oligoadenylate Synthetase/genetics — Biological Markers/metabolism — Cell Line — Cell Line, Tumor — Drug Delivery Systems/methods — Enzyme Induction/genetics — Gene Expression/genetics — Gene Silencing — Gene Transfer Techniques — Genetic Vectors/genetics — HeLa Cells/drug effects — Humans — Kidney/cytology/drug effects/embryology — Lentivirus/genetics/metabolism — Plasminogen Activator Inhibitor 2/biosynthesis/genetics/metabolism — RNA Stability — RNA, Antisense/administration & dosage/adverse effects/metabolism — RNA, Messenger/genetics/metabolism — RNA, Small Interfering/adverse effects/biosynthesis/metabolism — Transduction, Genetic/methods
Identifiers
PMID: 15291968
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Article (Published version) (1 MB) - public document Free access
Structures
Faculté de médecine / Section de médecine clinique / Département de médecine interne des spécialités
Faculté de médecine / Section de médecine fondamentale / Département de médecine génétique et développement
Citation
(ISO format) 		FISH, Richard, KRUITHOF, Egbert. Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors. In: BMC Molecular Biology, 2004, vol. 5, p. 9. https://archive-ouverte.unige.ch/unige:36194

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Deposited on : 2014-04-29

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