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Scientific article
English

Identification of post-mortem cerebrospinal fluid proteins as potential biomarkers of ischemia and neurodegeneration

Contributors

Lescuyer, Pierre; Allard, Laure; Zimmermann, Catherine; Burgess, Jennifer; Frutiger, Séverine; Burkhard, Pierre; Sanchez, Jean-Charles; Hochstrasser, Denis

Published in

Proteomics, vol. 4, no. 8, p. 2234-2241

Publication date

2004

Abstract

Only few biological markers are currently available for the routine diagnosis of brain damage-related disorders including cerebrovascular, dementia, and other neurodegenerative diseases. In this study, post-mortem cerebrospinal fluid samples were used as a model of massive brain insult to identify new markers potentially relevant for neurodegeneration. The protein pattern of this sample was compared to the one of cerebrospinal fluid from healthy subjects by two-dimensional gel electrophoresis. Using gel imaging, N-terminal microsequencing, mass spectrometry, and immunodetection techniques, we identified 13 differentially expressed proteins. Most of these proteins have been previously reported to be somehow associated with brain destruction or with the molecular mechanisms underlying certain neurodegenerative conditions. These data indicate that the identified proteins indeed represent potential biomarkers of brain damage. We recently showed that H-FABP, a protein highly homologous to E-FABP and A-FABP identified in this study, is a potential marker of Creutzfeldt-Jakob disease and stroke.

Keywords

  • Autopsy
  • Biological Markers
  • Cerebrospinal Fluid Proteins/analysis
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Immunoblotting
  • Ischemia/physiopathology
  • Mass Spectrometry
  • Molecular Sequence Data
  • Neurodegenerative Diseases/physiopathology
  • Peptide Mapping
  • Spinal Puncture

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

LESCUYER, Pierre et al. Identification of post-mortem cerebrospinal fluid proteins as potential biomarkers of ischemia and neurodegeneration. In: Proteomics, 2004, vol. 4, n° 8, p. 2234–2241. doi: 10.1002/pmic.200300822

Main files (1)

Article (Accepted version)

Identifiers

Journal ISSN

1615-9853
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Technical informations

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17/04/2014 12:21:00

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17/04/2014 12:21:00

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