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Title

Role of cellular effectors in the emergence of ventilation defects during allergic bronchoconstriction

Authors
Layachi, Skander
Porra, Liisa
Trouillet, Nathalie
Suhonen, Heikki
Sevestre, Henri
Suortti, Pekka
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Published in Journal of Applied Physiology. 2013, vol. 115, no. 7, p. 1057-64
Abstract It is not known whether local factors within the airway wall or parenchyma may influence the emergence and spatial distribution of ventilation defects (VDs), thereby modulating the dynamic system behavior of the lung during bronchoconstriction. We assessed the relationship between the distribution of cellular effectors and the emergence of defects in regional ventilation distribution following allergen challenge. We performed high-resolution K-edge subtraction (KES) synchrotron imaging during xenon inhalation and measured the forced oscillatory input impedance in ovalbumin (OVA)-sensitized Brown-Norway rats (n = 12) at baseline and repeatedly following OVA challenge. Histological slices with best anatomic matching to the computed tomographic images were stained with a modified May-Grunwald Giemsa and immunohistochemical staining with monoclonal anti-rat CD68, in six rats. Slides were digitized and total cells and eosinophils were counted in the walls of bronchi and vessels randomly selected within and outside of VDs on the basis of xenon-KES images. Ventilated alveolar area decreased and ventilation heterogeneity, Newtonian resistance, tissue damping, and elastance increased following OVA challenge. Eosinophil, total cell, and CD68+ counts were significantly higher in the bronchial and vascular walls within vs. outside of the VDs. The minimal central airway diameters during OVA-induced bronchoconstriction were correlated with eosinophil (R = -0.85; P = 0.031) and total cell densities (R = -0.82; P = 0.046) in the airway walls within the poorly ventilated zones. Our findings suggest that allergic airway inflammation is locally heterogeneous and is topographically associated with the local emergence of VDs following allergen challenge.
Identifiers
PMID: 23887899
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Research group Enfants et hyperréactivité bronchique (69)
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LAYACHI, Skander et al. Role of cellular effectors in the emergence of ventilation defects during allergic bronchoconstriction. In: Journal of Applied Physiology, 2013, vol. 115, n° 7, p. 1057-64. doi: 10.1152/japplphysiol.00844.2012 https://archive-ouverte.unige.ch/unige:35262

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Deposited on : 2014-04-02

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