UNIGE document Scientific Article
previous document  unige:35098  next document
add to browser collection
Title

Proteomic analysis of human substantia nigra identifies novel candidates involved in Parkinson's disease pathogenesis

Authors
Surini-Demiri, Maria
show hidden authors show all authors [1 - 9]
Published in Proteomics. 2014, vol. 14, no. 6, p. 784-94
Abstract Parkinson's disease (PD) pathology spreads throughout the brain following a region-specific process predominantly affecting the substantia nigra (SN) pars compacta. SN exhibits a progressive loss of dopaminergic neurons responsible for the major cardinal motor symptoms, along with the occurrence of Lewy bodies in the surviving neurons. To gain new insights into the underlying pathogenic mechanisms in PD, we studied postmortem nigral tissues dissected from pathologically confirmed PD cases (n = 5) and neurologically intact controls (n = 8). Using a high-throughput shotgun proteomic strategy, we simultaneously identified 1795 proteins with concomitant quantitative data. To date, this represents the most extensive catalog of nigral proteins. Of them, 204 proteins displayed significant expression level changes in PD patients versus controls. These were involved in novel or known pathogenic processes including mitochondrial dysfunction, oxidative stress, or cytoskeleton impairment. We further characterized four candidates that might be relevant to PD pathogenesis. We confirmed the differential expression of ferritin-L and seipin by Western blot and demonstrated the neuronal localization of gamma glutamyl hydrolase and nebulette by immunohistochemistry. Our preliminary findings suggest a role for nebulette overexpression in PD neurodegeneration, through mechanisms that may involve cytoskeleton dynamics disruption. All MS data have been deposited in the ProteomeXchange with identifier PXD000427 (http://proteomecentral.proteomexchange.org/dataset/PXD000427).
Identifiers
PMID: 24449343
Full text
Article (Author postprint) (754 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research groups Métastases du foie (657)
Groupe Pierre Burkhard (neurologie) (154)
Omics Panels of Tears Indicators for ocular and systemic Clinical Situations (957)
Citation
(ISO format)
LICKER, Virginie et al. Proteomic analysis of human substantia nigra identifies novel candidates involved in Parkinson's disease pathogenesis. In: Proteomics, 2014, vol. 14, n° 6, p. 784-94. https://archive-ouverte.unige.ch/unige:35098

188 hits

4 downloads

Update

Deposited on : 2014-03-28

Export document
Format :
Citation style :