Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma

Tyagi, Anuj Kumar; Pradier, Amandine; Baumer, Odile; Uppugunduri Satyanarayana, Chakradhara Rao; Huezo-Diaz, P; Posfay Barbe, Klara; Roosnek, Eddy Emile; Ansari Djaberi, Marc Georges

doi:10.1186/1743-422X-10-191

Scientific article

Open access

English

Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma

ContributorsTyagi, Anuj Kumar; Pradier, Amandine; Baumer, Odile; Uppugunduri Satyanarayana, Chakradhara Rao

; Huezo-Diaz, P; Posfay Barbe, Klara; Roosnek, Eddy Emile; Ansari Djaberi, Marc Georges

Published inVirology journal, vol. 10, 191

Publication date2013

Abstract

Quantification of titers of ubiquitous viruses such as Torque teno virus (TTV) that do not cause clinical symptoms might be helpful in assessing the immune status of an individual. We hereby describe the validation of a SYBR Green-based TTV quantification method for plasma samples.

Keywords

Adult
DNA Virus Infections/virology
Humans
Organic Chemicals/metabolism
Plasma/virology
Reproducibility of Results
Sensitivity and Specificity
Staining and Labeling/methods
Torque teno virus/isolation & purification
Viral Load/methods
Viremia/virology

Affiliation

Faculté de médecine / Section de médecine clinique / Département de pédiatrie, gynécologie et obstétrique

Research group

Citation (ISO format)

TYAGI, Anuj Kumar et al. Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma. In: Virology journal, 2013, vol. 10, p. 191. doi: 10.1186/1743-422X-10-191

Article (Published version)

CC BY

Identifiers

PID : unige:34846
DOI : 10.1186/1743-422X-10-191
PMID : 23758761

ISSN of the journal1743-422X

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Creation03/12/2014 2:17:00 PM

First validation03/12/2014 2:17:00 PM

Update time03/14/2023 9:01:48 PM

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Validation of SYBR Green based quantification assay for the detection of human Torque Teno virus titers from plasma

Quantification of titers of ubiquitous viruses such as Torque teno virus (TTV) that do not cause clinical symptoms might be helpful in assessing the immune status of an individual. We hereby describe the validation of a SYBR Green-based TTV quantification method for plasma samples.

Technical informations