UNIGE document Scientific Article
previous document  unige:34785  next document
add to browser collection
Title

Expansion and tissue infiltration of an allospecific CD4+CD25+CD45RO+IL-7Ralphahigh cell population in solid organ transplant recipients

Authors
Codarri, Laura
Vallotton, Laure
Venetz, Jean-Pierre
show hidden authors show all authors [1 - 10]
Published in Journal of Experimental Medicine. 2007, vol. 204, no. 7, p. 1533-41
Abstract It has been recently shown (Seddiki, N., B. Santner-Nanan, J. Martinson, J. Zaunders, S. Sasson, A. Landay, M. Solomon, W. Selby, S.I. Alexander, R. Nanan, et al. 2006. J. Exp. Med. 203:1693-1700.) that the expression of interleukin (IL) 7 receptor (R) alpha discriminates between two distinct CD4 T cell populations, both characterized by the expression of CD25, i.e. CD4 regulatory T (T reg) cells and activated CD4 T cells. T reg cells express low levels of IL-7Ralpha, whereas activated CD4 T cells are characterized by the expression of IL-7Ralpha(high). We have investigated the distribution of these two CD4 T cell populations in 36 subjects after liver and kidney transplantation and in 45 healthy subjects. According to a previous study (Demirkiran, A., A. Kok, J. Kwekkeboom, H.J. Metselaar, H.W. Tilanus, and L.J. van der Laan. 2005. Transplant. Proc. 37:1194-1196.), we observed that the T reg CD25(+)CD45RO(+)IL-7Ralpha(low) cell population was reduced in transplant recipients (P < 0.00001). Interestingly, the CD4(+)CD25(+)CD45RO(+)IL-7Ralpha(high) cell population was significantly increased in stable transplant recipients compared with healthy subjects (P < 0.00001), and the expansion of this cell population was even greater in patients with documented humoral chronic rejection compared with stable transplant recipients (P < 0.0001). The expanded CD4(+)CD25(+)CD45RO(+)IL-7Ralpha(high) cell population contained allospecific CD4 T cells and secreted effector cytokines such as tumor necrosis factor alpha and interferon gamma, thus potentially contributing to the mechanisms of chronic rejection. More importantly, CD4(+)IL-7Ralpha(+)and CD25(+)IL-7Ralpha(+) cells were part of the T cell population infiltrating the allograft of patients with a documented diagnosis of chronic humoral rejection. These results indicate that the CD4(+)CD25(+)IL-7Ralpha(+) cell population may represent a valuable, sensitive, and specific marker to monitor allospecific CD4 T cell responses both in blood and in tissues after organ transplantation.
Keywords Antigens, CD/immunologyAntigens, CD4/immunologyAntigens, CD45/immunologyCD4-Positive T-Lymphocytes/immunologyCytokines/secretionFlow CytometryHumansImmunosuppressionInterleukin-2 Receptor alpha Subunit/immunologyKidney Transplantation/immunologyLiver Transplantation/immunologyReceptors, Interleukin-7/immunologyT-Lymphocytes/immunology
Identifiers
PMID: 17591854
Full text
Article (Published version) (1.8 MB) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research groups Cultures cellulaires et transplantations (519)
Hépatologie chirurgicale (327)
Citation
(ISO format)
CODARRI, Laura et al. Expansion and tissue infiltration of an allospecific CD4+CD25+CD45RO+IL-7Ralphahigh cell population in solid organ transplant recipients. In: Journal of Experimental Medicine, 2007, vol. 204, n° 7, p. 1533-41. https://archive-ouverte.unige.ch/unige:34785

185 hits

0 download

Update

Deposited on : 2014-03-14

Export document
Format :
Citation style :