Scientific article
Open access

A high-resolution anatomical atlas of the transcriptome in the mouse embryo

CollaboratorsReymond, Alexandre
Published inPLoS biology, vol. 9, no. 1, e1000582
Publication date2011

Ascertaining when and where genes are expressed is of crucial importance to understanding or predicting the physiological role of genes and proteins and how they interact to form the complex networks that underlie organ development and function. It is, therefore, crucial to determine on a genome-wide level, the spatio-temporal gene expression profiles at cellular resolution. This information is provided by colorimetric RNA in situ hybridization that can elucidate expression of genes in their native context and does so at cellular resolution. We generated what is to our knowledge the first genome-wide transcriptome atlas by RNA in situ hybridization of an entire mammalian organism, the developing mouse at embryonic day 14.5. This digital transcriptome atlas, the Eurexpress atlas (http://www.eurexpress.org), consists of a searchable database of annotated images that can be interactively viewed. We generated anatomy-based expression profiles for over 18,000 coding genes and over 400 microRNAs. We identified 1,002 tissue-specific genes that are a source of novel tissue-specific markers for 37 different anatomical structures. The quality and the resolution of the data revealed novel molecular domains for several developing structures, such as the telencephalon, a novel organization for the hypothalamus, and insight on the Wnt network involved in renal epithelial differentiation during kidney development. The digital transcriptome atlas is a powerful resource to determine co-expression of genes, to identify cell populations and lineages, and to identify functional associations between genes relevant to development and disease.

  • Animals
  • Atlases as Topic
  • Databases, Genetic
  • Embryo, Mammalian
  • Gene Expression Profiling
  • Internet
  • Mice/anatomy & histology/embryology/genetics
  • Mice, Inbred C57BL
  • Organ Specificity
Citation (ISO format)
DIEZ-ROUX, Graciana. A high-resolution anatomical atlas of the transcriptome in the mouse embryo. In: PLoS biology, 2011, vol. 9, n° 1, p. e1000582. doi: 10.1371/journal.pbio.1000582
Main files (1)
Article (Published version)
ISSN of the journal1544-9173

Technical informations

Creation02/19/2014 4:42:00 PM
First validation02/19/2014 4:42:00 PM
Update time03/14/2023 9:00:44 PM
Status update03/14/2023 9:00:43 PM
Last indexation01/16/2024 9:21:28 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack