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Network-guided analysis of genes with altered somatic copy number and gene expression reveals pathways commonly perturbed in metastatic melanoma

Valsesia, Armand
Rimoldi, Donata
Martinet, Danielle
Ibberson, Mark
Benaglio, Paola
Quadroni, Manfredo
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Published in PLOS ONE. 2011, vol. 6, no. 4, e18369
Abstract Cancer genomes frequently contain somatic copy number alterations (SCNA) that can significantly perturb the expression level of affected genes and thus disrupt pathways controlling normal growth. In melanoma, many studies have focussed on the copy number and gene expression levels of the BRAF, PTEN and MITF genes, but little has been done to identify new genes using these parameters at the genome-wide scale. Using karyotyping, SNP and CGH arrays, and RNA-seq, we have identified SCNA affecting gene expression ('SCNA-genes') in seven human metastatic melanoma cell lines. We showed that the combination of these techniques is useful to identify candidate genes potentially involved in tumorigenesis. Since few of these alterations were recurrent across our samples, we used a protein network-guided approach to determine whether any pathways were enriched in SCNA-genes in one or more samples. From this unbiased genome-wide analysis, we identified 28 significantly enriched pathway modules. Comparison with two large, independent melanoma SCNA datasets showed less than 10% overlap at the individual gene level, but network-guided analysis revealed 66% shared pathways, including all but three of the pathways identified in our data. Frequently altered pathways included WNT, cadherin signalling, angiogenesis and melanogenesis. Additionally, our results emphasize the potential of the EPHA3 and FRS2 gene products, involved in angiogenesis and migration, as possible therapeutic targets in melanoma. Our study demonstrates the utility of network-guided approaches, for both large and small datasets, to identify pathways recurrently perturbed in cancer.
Keywords Cell Line, TumorComparative Genomic HybridizationDNA Copy Number Variations/geneticsDatabases, GeneticGene Expression Regulation, NeoplasticGene Regulatory Networks/geneticsGenes, Neoplasm/geneticsHumansIn Situ Hybridization, FluorescenceKaryotypingMelanoma/genetics/pathologyNeoplasm MetastasisPolymorphism, Single Nucleotide/geneticsProto-Oncogene Proteins c-mdm2/geneticsRNA, Messenger/genetics/metabolismSignal Transduction/genetics
PMID: 21494657
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Research group Pathologie Moléculaire de la Trisomie 21 et le Génome Humain (248)
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VALSESIA, Armand et al. Network-guided analysis of genes with altered somatic copy number and gene expression reveals pathways commonly perturbed in metastatic melanoma. In: PLOS ONE, 2011, vol. 6, n° 4, p. e18369. doi: 10.1371/journal.pone.0018369 https://archive-ouverte.unige.ch/unige:34661

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Deposited on : 2014-03-07

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