UNIGE document Scientific Article
previous document  unige:34649  next document
add to browser collection

Evidence for transcript networks composed of chimeric RNAs in human cells

Djebali, Sarah
Lagarde, Julien
Kapranov, Philipp
Lacroix, Vincent
CollaborationWith : Borel, Christelle / Ucla, Catherine / Martin, David / Reymond, Alexandre / Antonarakis, Stylianos
Published in PloS ONE. 2012, vol. 7, no. 1, e28213
Abstract The classic organization of a gene structure has followed the Jacob and Monod bacterial gene model proposed more than 50 years ago. Since then, empirical determinations of the complexity of the transcriptomes found in yeast to human has blurred the definition and physical boundaries of genes. Using multiple analysis approaches we have characterized individual gene boundaries mapping on human chromosomes 21 and 22. Analyses of the locations of the 5' and 3' transcriptional termini of 492 protein coding genes revealed that for 85% of these genes the boundaries extend beyond the current annotated termini, most often connecting with exons of transcripts from other well annotated genes. The biological and evolutionary importance of these chimeric transcripts is underscored by (1) the non-random interconnections of genes involved, (2) the greater phylogenetic depth of the genes involved in many chimeric interactions, (3) the coordination of the expression of connected genes and (4) the close in vivo and three dimensional proximity of the genomic regions being transcribed and contributing to parts of the chimeric RNAs. The non-random nature of the connection of the genes involved suggest that chimeric transcripts should not be studied in isolation, but together, as an RNA network.
Keywords AlgorithmsCells/metabolismChimerin Proteins/chemistry/geneticsChromosomes, Human, Pair 1/geneticsFemaleGene Expression ProfilingGene Regulatory Networks/genetics/physiologyHumansMaleMicroarray Analysis/methodsModels, BiologicalNucleic Acid Amplification Techniques/methodsRNA/genetics/physiologyRNA Isoforms/chemistry/genetics/metabolismTranscription, Genetic/geneticsTranscriptome/physiologyValidation Studies as Topic
PMID: 22238572
Full text
Article (Published version) (1.7 MB) - public document Free access
Research group Pathologie Moléculaire de la Trisomie 21 et le Génome Humain (248)
(ISO format)
DJEBALI, Sarah et al. Evidence for transcript networks composed of chimeric RNAs in human cells. In: PLOS ONE, 2012, vol. 7, n° 1, p. e28213. doi: 10.1371/journal.pone.0028213 https://archive-ouverte.unige.ch/unige:34649

1024 hits



Deposited on : 2014-03-07

Export document
Format :
Citation style :