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Title

Exome sequencing identifies putative drivers of progression of transient myeloproliferative disorder to AMKL in infants with Down syndrome

Authors
Giarin, Emanuela
Basso, Giuseppe
Hoischen, Alexander
Veltman, Joris A
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Published in Blood. 2013, vol. 122, no. 4, p. 554-61
Abstract Some neonates with Down syndrome (DS) are diagnosed with self-regressing transient myeloproliferative disorder (TMD), and 20% to 30% of those progress to acute megakaryoblastic leukemia (AMKL). We performed exome sequencing in 7 TMD/AMKL cases and copy-number analysis in these and 10 additional cases. All TMD/AMKL samples contained GATA1 mutations. No exome-sequenced TMD/AMKL sample had other recurrently mutated genes. However, 2 of 5 TMD cases, and all AMKL cases, showed mutations/deletions other than GATA1, in genes proven as transformation drivers in non-DS leukemia (EZH2, APC, FLT3, JAK1, PARK2-PACRG, EXT1, DLEC1, and SMC3). One patient at the TMD stage revealed 2 clonal expansions with different GATA1 mutations, of which 1 clone had an additional driver mutation. Interestingly, it was the other clone that gave rise to AMKL after accumulating mutations in 7 other genes. Data suggest that GATA1 mutations alone are sufficient for clonal expansions, and additional driver mutations at the TMD stage do not necessarily predict AMKL progression. Later in infancy, leukemic progression requires "third-hit driver" mutations/somatic copy-number alterations found in non-DS leukemias. Putative driver mutations affecting WNT (wingless-related integration site), JAK-STAT (Janus kinase/signal transducer and activator of transcription), or MAPK/PI3K (mitogen-activated kinase/phosphatidylinositol-3 kinase) pathways were found in all cases, aberrant activation of which converges on overexpression of MYC.
Keywords Cell Transformation, Neoplastic/geneticsDisease ProgressionDown Syndrome/complications/geneticsExome/geneticsGenetic Predisposition to Disease/geneticsGenome-Wide Association StudyGenomic Instability/geneticsHigh-Throughput Nucleotide Sequencing/methodsHumansInfantInfant, NewbornLeukemia, Megakaryoblastic, Acute/complications/genetics/pathologyMicroarray AnalysisMyeloproliferative Disorders/complications/genetics/pathologyPolymorphism, Single NucleotideTranscriptome
Identifiers
PMID: 23733339
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Research group Pathologie Moléculaire de la Trisomie 21 et le Génome Humain (248)
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NIKOLAEV, Sergey Igorievich et al. Exome sequencing identifies putative drivers of progression of transient myeloproliferative disorder to AMKL in infants with Down syndrome. In: Blood, 2013, vol. 122, n° 4, p. 554-61. https://archive-ouverte.unige.ch/unige:34516

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Deposited on : 2014-02-28

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