UNIGE document Scientific Article
previous document  unige:33926  next document
add to browser collection
Title

Nitric oxide mediates local activity-dependent excitatory synapse development

Authors
Nikonenko, Alexander
Michurina, Tatyana V
Enikolopov, Grigori
Published in Proceedings of the National Academy of Sciences. 2013, vol. 110, no. 44, p. E4142-51
Abstract Learning related paradigms play an important role in shaping the development and specificity of synaptic networks, notably by regulating mechanisms of spine growth and pruning. The molecular events underlying these synaptic rearrangements remain poorly understood. Here we identify NO signaling as a key mediator of activity-dependent excitatory synapse development. We find that chronic blockade of NO production in vitro and in vivo interferes with the development of hippocampal and cortical excitatory spine synapses. The effect results from a selective loss of activity-mediated spine growth mechanisms and is associated with morphological and functional alterations of remaining synapses. These effects of NO are mediated by a cGMP cascade and can be reproduced or prevented by postsynaptic expression of vasodilator-stimulated phosphoprotein phospho-mimetic or phospho-resistant mutants. In vivo analyses show that absence of NO prevents the increase in excitatory synapse density induced by environmental enrichment and interferes with the formation of local clusters of excitatory synapses. We conclude that NO plays an important role in regulating the development of excitatory synapses by promoting local activity-dependent spine-growth mechanisms.
Identifiers
PMID: 24127602
Full text
Article (Published version) (1.6 MB) - public document Free access
Structures
Research group Plasticité des synapses excitatrices (76)
Citation
(ISO format)
NIKONENKO, Iryna et al. Nitric oxide mediates local activity-dependent excitatory synapse development. In: Proceedings of the National Academy of Sciences, 2013, vol. 110, n° 44, p. E4142-51. https://archive-ouverte.unige.ch/unige:33926

205 hits

108 downloads

Update

Deposited on : 2014-01-31

Export document
Format :
Citation style :