Scientific article
English

Mitochondrial matrix calcium is an activating signal for hormone secretion

Published inCell metabolism, vol. 13, no. 5, p. 601-611
Publication date2011
Abstract

Mitochondrial Ca(2+) signals have been proposed to accelerate oxidative metabolism and ATP production to match Ca(2+)-activated energy-consuming processes. Efforts to understand the signaling role of mitochondrial Ca(2+) have been hampered by the inability to manipulate matrix Ca(2+) without directly altering cytosolic Ca(2+). We were able to selectively buffer mitochondrial Ca(2+) rises by targeting the Ca(2+)-binding protein S100G to the matrix. We find that matrix Ca(2+) controls signal-dependent NAD(P)H formation, respiration, and ATP changes in intact cells. Furthermore, we demonstrate that matrix Ca(2+) increases are necessary for the amplification of sustained glucose-dependent insulin secretion in β cells. Through the regulation of NAD(P)H in adrenal glomerulosa cells, matrix Ca(2+) also acts as a positive signal in reductive biosynthesis, which stimulates aldosterone secretion. Our dissection of cytosolic and mitochondrial Ca(2+) signals reveals the physiological importance of matrix Ca(2+) in energy metabolism required for signal-dependent hormone secretion.

Keywords
  • Aldosterone/secretion
  • Animals
  • Calbindins
  • Calcium/metabolism
  • Cells, Cultured
  • Cytosol/metabolism
  • Glucose/metabolism
  • Immunoenzyme Techniques
  • Insulin/metabolism
  • Insulin-Secreting Cells/metabolism
  • Membrane Potential, Mitochondrial
  • Mitochondria/metabolism
  • NADP/metabolism
  • Oxygen Consumption
  • Rats
  • S100 Calcium Binding Protein G/metabolism
  • Zona Glomerulosa/cytology/metabolism
Citation (ISO format)
WIEDERKEHR, Andreas et al. Mitochondrial matrix calcium is an activating signal for hormone secretion. In: Cell metabolism, 2011, vol. 13, n° 5, p. 601–611. doi: 10.1016/j.cmet.2011.03.015
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Article (Published version)
accessLevelRestricted
Identifiers
Journal ISSN1550-4131
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