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Identification of brain cell death associated proteins in human post-mortem cerebrospinal fluid

Michel, Philippe
Rossier, Joël S
Reymond, Frédéric
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Published in Journal of proteome research. 2006, vol. 5, no. 7, p. 1674-81
Abstract Following any form of brain insult, proteins are released from damaged tissues into the cerebrospinal fluid (CSF). This body fluid is therefore an ideal sample to use in the search for biomarkers of neurodegenerative disorders and brain damage. In this study, we used human post-mortem CSF as a model of massive brain injury and cell death for the identification of such protein markers. Pooled post-mortem CSF samples were analyzed using a protocol that combined immunoaffinity depletion of abundant CSF proteins, off-gel electrophoresis, SDS-PAGE and protein identification by LC-MS/MS. A total of 299 proteins were identified, of which 172 proteins were not previously described to be present in CSF. Of these 172 proteins, more than 75% have been described as intracellular proteins suggesting that they were released from damaged cells. Immunoblots of a number of proteins were performed on individual post-mortem CSF samples and confirmed elevated concentrations in post-mortem CSF compared to ante-mortem CSF. Interestingly, among the proteins specifically identified in the post-mortem CSF, several have been previously described as biochemical markers of brain damage.
Keywords Biological Markers/cerebrospinal fluidBrain Injuries/pathologyCell DeathCerebrospinal Fluid Proteins/analysis/classificationHumansNeurodegenerative Diseases/cerebrospinal fluidProteins/chemistry/classificationProteomics/methods
PMID: 16823975
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Research groups Groupe Pierre Burkhard (neurologie) (154)
Groupe de Protéomique biomédicale (635)
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BURGESS, Jennifer et al. Identification of brain cell death associated proteins in human post-mortem cerebrospinal fluid. In: Journal of proteome research, 2006, vol. 5, n° 7, p. 1674-81. doi: 10.1021/pr060160v https://archive-ouverte.unige.ch/unige:33533

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Deposited on : 2014-01-22

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