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Title

A novel strategy using MASCOT Distiller for analysis of cleavable isotope-coded affinity tag data to quantify protein changes in plasma

Authors
Leung, Kit-Yi
Campbell, James
Byers, Helen L
Ward, Malcolm A
Published in Proteomics. 2005, vol. 5, no. 12, p. 3040-4
Abstract A novel strategy consisting of cleavable Isotope-Coded Affinity Tag (cICAT) combined with MASCOT Distiller was evaluated as a tool for the quantification of proteins in "abnormal" patient plasma, prepared by pooling samples from patients with acute stroke. Quantification of all light and heavy cICAT-labelled peptide ion pairs was obtained using MASCOT Distiller combined with a proprietary software. Peptides displaying differences were selected for identification by MS. These preliminary results show the promise of our approach to identify potential biomarkers.
Keywords AlgorithmsAnimalsBiological MarkersBlood Proteins/chemistryComputational Biology/methodsDatabases, ProteinHumansImmunoglobulin G/chemistryIonsIsotopesMass Spectrometry/methodsPeptide MappingPeptides/chemistryProteins/chemistryProteomeProteomics/methodsSoftwareTime FactorsUp-Regulation
Identifiers
PMID: 16041675
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Article (Published version) (126 Kb) - document accessible for UNIGE members only Limited access to UNIGE
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Research group Groupe de Protéomique biomédicale (635)
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LEUNG, Kit-Yi et al. A novel strategy using MASCOT Distiller for analysis of cleavable isotope-coded affinity tag data to quantify protein changes in plasma. In: Proteomics, 2005, vol. 5, n° 12, p. 3040-4. https://archive-ouverte.unige.ch/unige:33526

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Deposited on : 2014-01-22

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