en
Scientific article
English

Truncated cystatin C in cerebrospiral fluid: Technical [corrected] artefact or biological process?

Published inProteomics, vol. 5, no. 12, p. 3060-3065
Publication date2005
Abstract

Cystatin C, a low molecular weight cysteine proteinase inhibitor present in human body fluids at physiological concentrations, is more expressed in cerebrospinal fluid (CSF) than in plasma. Mass spectrometric characterization showed that after 3 months of storage of human CSF at -20 degrees C, cystatin C was cleaved in the peptide bond between R8 and L9 and lost its eight N-termini amino acids, whereas this cleavage did not occur when stored at -80 degrees C. This truncation occurred in all CSF samples studied irrespective of the underlying neurological status, indicating a storage-related artefact rather than a physiological or pathological processing of the protein. These results stress the importance of optimal preanalytical storage conditions of any sample prior to proteomics studies.

Keywords
  • Artifacts
  • Cystatin C
  • Cystatins/cerebrospinal fluid/chemistry
  • Cysteine Proteinase Inhibitors/chemistry
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Mass Spectrometry/methods
  • Protein Array Analysis
  • Protein Structure, Tertiary
  • Proteins/chemistry
  • Proteomics/methods
  • Specimen Handling
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Temperature
  • Time Factors
Citation (ISO format)
CARRETTE, Odile et al. Truncated cystatin C in cerebrospiral fluid: Technical [corrected] artefact or biological process? In: Proteomics, 2005, vol. 5, n° 12, p. 3060–3065. doi: 10.1002/pmic.200402039
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal1615-9853
513views
0downloads

Technical informations

Creation11/15/2013 12:59:00 PM
First validation11/15/2013 12:59:00 PM
Update time03/14/2023 8:52:46 PM
Status update03/14/2023 8:52:46 PM
Last indexation01/16/2024 8:58:42 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack