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Truncated cystatin C in cerebrospiral fluid: Technical [corrected] artefact or biological process?

Hughes, Severine
Published in Proteomics. 2005, vol. 5, no. 12, p. 3060-5
Abstract Cystatin C, a low molecular weight cysteine proteinase inhibitor present in human body fluids at physiological concentrations, is more expressed in cerebrospinal fluid (CSF) than in plasma. Mass spectrometric characterization showed that after 3 months of storage of human CSF at -20 degrees C, cystatin C was cleaved in the peptide bond between R8 and L9 and lost its eight N-termini amino acids, whereas this cleavage did not occur when stored at -80 degrees C. This truncation occurred in all CSF samples studied irrespective of the underlying neurological status, indicating a storage-related artefact rather than a physiological or pathological processing of the protein. These results stress the importance of optimal preanalytical storage conditions of any sample prior to proteomics studies.
Keywords ArtifactsCystatin CCystatins/cerebrospinal fluid/chemistryCysteine Proteinase Inhibitors/chemistryElectrophoresis, Polyacrylamide GelHumansMass Spectrometry/methodsProtein Array AnalysisProtein Structure, TertiaryProteins/chemistryProteomics/methodsSpecimen HandlingSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationTemperatureTime Factors
PMID: 16021609
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Research group Groupe de Recherche Translationnelle en Biomarqueurs (635)
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CARRETTE, Odile et al. Truncated cystatin C in cerebrospiral fluid: Technical [corrected] artefact or biological process?. In: Proteomics, 2005, vol. 5, n° 12, p. 3060-5. doi: 10.1002/pmic.200402039 https://archive-ouverte.unige.ch/unige:33524

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Deposited on : 2014-01-22

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