Article (Published version) (158 Kb) - Limited access to UNIGE
Truncated cystatin C in cerebrospiral fluid: Technical [corrected] artefact or biological process?
|Published in||Proteomics. 2005, vol. 5, no. 12, p. 3060-5|
|Abstract||Cystatin C, a low molecular weight cysteine proteinase inhibitor present in human body fluids at physiological concentrations, is more expressed in cerebrospinal fluid (CSF) than in plasma. Mass spectrometric characterization showed that after 3 months of storage of human CSF at -20 degrees C, cystatin C was cleaved in the peptide bond between R8 and L9 and lost its eight N-termini amino acids, whereas this cleavage did not occur when stored at -80 degrees C. This truncation occurred in all CSF samples studied irrespective of the underlying neurological status, indicating a storage-related artefact rather than a physiological or pathological processing of the protein. These results stress the importance of optimal preanalytical storage conditions of any sample prior to proteomics studies.|
|Keywords||Artifacts — Cystatin C — Cystatins/cerebrospinal fluid/chemistry — Cysteine Proteinase Inhibitors/chemistry — Electrophoresis, Polyacrylamide Gel — Humans — Mass Spectrometry/methods — Protein Array Analysis — Protein Structure, Tertiary — Proteins/chemistry — Proteomics/methods — Specimen Handling — Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization — Temperature — Time Factors|
|Research group||Groupe de Recherche Translationnelle en Biomarqueurs (635)|
|CARRETTE, Odile et al. Truncated cystatin C in cerebrospiral fluid: Technical [corrected] artefact or biological process?. In: Proteomics, 2005, vol. 5, n° 12, p. 3060-5. doi: 10.1002/pmic.200402039 https://archive-ouverte.unige.ch/unige:33524|