en
Scientific article
English

The translationally controlled tumor protein is a novel calcium binding protein of the human placenta and regulates calcium handling in trophoblast cells

Published inBiology of reproduction, vol. 73, no. 4, p. 745-751
Publication date2005
Abstract

The translationally controlled tumor protein (TPT1, also known as TCTP) is a highly conserved, abundantly expressed protein found in mammals as well as in a wide range of other organisms of both the animal and plant kingdom. Initially considered as a growth-related protein, later studies showed TPT1 is endowed with multiple biological activities, including calcium binding. The present study aimed to evaluate the expression of TPT1 in the human placenta and to examine the functional role of the protein in the calcium binding and homeostasis of trophoblast cells. Samples were analyzed by Western blot, reverse transcription-polymerase chain reaction and immunohistochemistry. The effect of TPT1 knockdown by small interfering RNA (siRNA) on calcium uptake and buffering was assessed in the HTR-8/SVneo cell line. TPT1 protein and mRNA were detected in first-trimester and term placenta. In the tissue, TPT1 was localized in the villous trophoblast. TPT1 expression significantly increased during gestation, with the higher protein and mRNA levels reached at term. Recombinant placental TPT1 bound calcium in vitro, while downregulation of the protein levels by siRNA in HTR-8/SVneo cells was associated with a reduced cellular calcium-uptake activity and buffering capacity. These data demonstrate, for the first time, the expression of TPT1 in the human placenta and support a direct role of the protein in placental calcium transport.

Keywords
  • Biological Transport
  • Blotting, Western
  • Calcium/metabolism
  • Cells, Cultured
  • Female
  • Gene Expression Regulation, Developmental
  • Humans
  • Neoplasm Proteins/genetics/metabolism
  • Nuclear Proteins/genetics/metabolism
  • Placenta/metabolism
  • Pregnancy
  • Pregnancy Trimesters
  • RNA, Small Interfering
  • Reverse Transcriptase Polymerase Chain Reaction/methods
  • Trophoblasts/metabolism
  • Tumor Markers, Biological
Citation (ISO format)
ARCURI, Felice et al. The translationally controlled tumor protein is a novel calcium binding protein of the human placenta and regulates calcium handling in trophoblast cells. In: Biology of reproduction, 2005, vol. 73, n° 4, p. 745–751. doi: 10.1095/biolreprod.105.042077
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0006-3363
519views
0downloads

Technical informations

Creation11/15/2013 1:02:00 PM
First validation11/15/2013 1:02:00 PM
Update time03/14/2023 8:52:46 PM
Status update03/14/2023 8:52:45 PM
Last indexation01/16/2024 8:58:37 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack