UNIGE document Scientific Article
previous document  unige:33517  next document
add to browser collection
Title

Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect

Authors
Zimmermann-Ivol, Catherine G
Di Dio, Joel
Vaezzadeh, Ali R
Amez-Droz, Michel
Cochard, Roland
show hidden authors show all authors [1 - 10]
Published in Rapid Communications in Mass Spectrometry. 2005, vol. 19, no. 5, p. 605-10
Abstract Acquisition of tandem mass spectra from peptides or other analytes deposited on non-conductive membranes is inhibited on instruments combining matrix-assisted laser desorption/ionization with tandem time-of-flight analyzers (MALDI-TOF/TOF) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI-TOF/TOF systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry.
Keywords AdsorptionCoated Materials, Biocompatible/analysis/chemistryElectric ConductivityGold/chemistryMembranes, ArtificialPeptide Mapping/methodsProtein BindingProteins/analysis/chemistrySpectrometry, Mass, Electrospray Ionization/methodsSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
Identifiers
PMID: 15685683
Full text
Article (Published version) (269 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Groupe de Protéomique biomédicale (635)
Citation
(ISO format)
SCHERL, Alexander et al. Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect. In: Rapid Communications in Mass Spectrometry, 2005, vol. 19, n° 5, p. 605-10. https://archive-ouverte.unige.ch/unige:33517

129 hits

0 download

Update

Deposited on : 2014-01-22

Export document
Format :
Citation style :