Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect

Scherl, Alexander; Zimmermann-Ivol, Catherine G; Di Dio, Joel; Vaezzadeh, Ali R; Binz, Pierre-Alain; Amez-Droz, Michel; Cochard, Roland; Sanchez, Jean-Charles; Glückmann, Matthias; Hochstrasser, Denis

doi:10.1002/rcm.1831

Scientific article

English

Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect

ContributorsScherl, Alexander; Zimmermann-Ivol, Catherine G; Di Dio, Joel; Vaezzadeh, Ali R; Binz, Pierre-Alain; Amez-Droz, Michel; Cochard, Roland; Sanchez, Jean-Charles

; Glückmann, Matthias; Hochstrasser, Denis

Published inRCM. Rapid communications in mass spectrometry, vol. 19, no. 5, p. 605-610

Publication date2005

Abstract

Acquisition of tandem mass spectra from peptides or other analytes deposited on non-conductive membranes is inhibited on instruments combining matrix-assisted laser desorption/ionization with tandem time-of-flight analyzers (MALDI-TOF/TOF) due to a charging effect. A thin layer of gold renders the membrane conductive. This allows adequate data acquisition on MALDI-TOF/TOF systems. Therefore, this methodology extends the capacity of the molecular scanner concept to tandem mass spectrometry.

Keywords

Adsorption
Coated Materials, Biocompatible/analysis/chemistry
Electric Conductivity
Gold/chemistry
Membranes, Artificial
Peptide Mapping/methods
Protein Binding
Proteins/analysis/chemistry
Spectrometry, Mass, Electrospray Ionization/methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods

Affiliation entities

Faculté de médecine / Section de médecine fondamentale / Département de biologie structurale et bioinformatique

Research groups

Groupe de Recherche Translationnelle en Biomarqueurs (635)

Citation (ISO format)

SCHERL, Alexander et al. Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect. In: RCM. Rapid communications in mass spectrometry, 2005, vol. 19, n° 5, p. 605–610. doi: 10.1002/rcm.1831

Article (Published version)

Identifiers

PID : unige:33517
DOI : 10.1002/rcm.1831
PMID : 15685683

Journal ISSN0951-4198

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Gold coating of non-conductive membranes before matrix-assisted laser desorption/ionization tandem mass spectrometric analysis prevents charging effect

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