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Inhibition of insulin secretion by betagranin, an N-terminal chromogranin A fragment

Schmid, Gerhard M
Caille, Dorothée
Wargent, Ed
O'Dowd, Jacqueline
Cawthorne, Michael A
Published in Journal of Biological Chemistry. 2007, vol. 282, no. 17, p. 12717-24
Abstract Betagranin, an N-terminal fragment of chromogranin A, results from a proteolytic processing, and is co-secreted with insulin. While other chromogranin A-derived peptides negatively modulate hormone secretion, the role of betagranin in pancreatic beta-cells is so far unknown. We have recently shown that pancreatic islet betagranin levels are down-regulated in obese, leptin-deficient mice. In the present study, we have investigated the distribution of betagranin in primary mouse islets and cells of the MIN6 line and have evaluated its effects on insulin secretion. We showed that betagranin co-localizes with insulin within secretory granules and strongly inhibited insulin secretion in response to both glucose and potassium, by blocking the influx of calcium. The data demonstrated a hitherto unknown inhibitory effect of betagranin on insulin secretion.
Keywords AnimalsCalcium Signaling/drug effectsCell LineChromogranins/pharmacologyFemaleGlucose/metabolism/pharmacologyInsulin/secretionIslets of Langerhans/metabolism/ultrastructureMicePotassium/metabolism/pharmacologySweetening Agents/pharmacology
PMID: 17289672
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Research group Groupe de Protéomique biomédicale (635)
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SCHMID, Gerhard M et al. Inhibition of insulin secretion by betagranin, an N-terminal chromogranin A fragment. In: Journal of Biological Chemistry, 2007, vol. 282, n° 17, p. 12717-24. https://archive-ouverte.unige.ch/unige:33513

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Deposited on : 2014-01-22

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