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Title

Role of seipin in lipid droplet morphology and hepatitis C virus life cycle

Authors
Fauvelle, Catherine
Boldanova, Tujana
Bartosch, Birke
Minehira, Kaori
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Published in Journal of General Virology. 2013, vol. 94, no. Pt 10, p. 2208-14
Abstract Infectious hepatitis C virus (HCV) particle assembly starts at the surface of lipid droplets, cytoplasmic organelles responsible for neutral fat storage. We analysed the relationship between HCV and seipin, a protein involved in lipid droplet maturation. Although seipin overexpression did not affect the total mean volume occupied by lipid droplets nor the total triglyceride and cholesterol ester levels per cell, it caused an increase in the mean diameter of lipid droplets by 60 %, while decreasing their total number per cell. The latter two effects combined resulted in a 34 % reduction of the total outer surface area of lipid droplets per cell, with a proportional decrease in infectious viral particle production, probably due to a defect in particle assembly. These results suggest that the available outer surface of lipid droplets is a critical factor for HCV release, independent of the neutral lipid content of the cell.
Keywords Cell Line, TumorGTP-Binding Protein gamma Subunits/genetics/metabolismGene Expression Regulation/physiologyHepacivirus/physiologyHumansLipids/chemistryVirus InternalizationVirus Replication/physiology
Identifiers
PMID: 23907395
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Research group Etudes et traitement de l'hépatite C et B (554)
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CLEMENT, Sophie et al. Role of seipin in lipid droplet morphology and hepatitis C virus life cycle. In: Journal of General Virology, 2013, vol. 94, n° Pt 10, p. 2208-14. https://archive-ouverte.unige.ch/unige:33507

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Deposited on : 2014-01-21

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