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Scientific article
English

Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses

Published inApplied and environmental microbiology, vol. 73, no. 17, p. 5653-5656
Publication date2007
Abstract

Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [(13)C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence.

Keywords
  • Amino Acid Sequence
  • Bacterial Proteins/chemistry/metabolism
  • Bacteriological Techniques
  • Bifidobacterium/growth & development/metabolism
  • Carbon Isotopes/chemistry/metabolism
  • Culture Media
  • Isotope Labeling/methods
  • Leucine/metabolism
  • Molecular Sequence Data
  • Peptides/chemistry/metabolism
  • Proteome
  • Proteomics/methods
Citation (ISO format)
COUTE, Yohann et al. Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses. In: Applied and environmental microbiology, 2007, vol. 73, n° 17, p. 5653–5656. doi: 10.1128/AEM.00667-07
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Article (Published version)
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Identifiers
ISSN of the journal0099-2240
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