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Title

Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses

Authors
Hernandez, Céline
Margolles, Abelardo
Published in Applied and Environmental Microbiology. 2007, vol. 73, no. 17, p. 5653-6
Abstract Stable isotope labeling of amino acids in cell culture was used for Bifidobacterium longum. A comprehensive proteomic strategy was developed and validated by designing an appropriate semidefined medium that allows stable replacement of natural leucine by [(13)C6]leucine. Using this strategy, proteins having variations of at least 50% in their expression rates can be quantified with great confidence.
Keywords Amino Acid SequenceBacterial Proteins/chemistry/metabolismBacteriological TechniquesBifidobacterium/growth & development/metabolismCarbon Isotopes/chemistry/metabolismCulture MediaIsotope Labeling/methodsLeucine/metabolismMolecular Sequence DataPeptides/chemistry/metabolismProteomeProteomics/methods
Identifiers
PMID: 17601805
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Research group Groupe de Protéomique biomédicale (635)
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(ISO format)
COUTE, Yohann et al. Labeling of Bifidobacterium longum cells with 13C-substituted leucine for quantitative proteomic analyses. In: Applied and Environmental Microbiology, 2007, vol. 73, n° 17, p. 5653-6. https://archive-ouverte.unige.ch/unige:33483

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Deposited on : 2014-01-21

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