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In vivo metabolic profiling of glioma-initiating cells using proton magnetic resonance spectroscopy at 14.1 Tesla

Mlynárik, Vladimír
Cudalbu, Cristina
Published in NMR in biomedicine. 2012, vol. 25, no. 4, p. 506-13
Abstract In the last decade, evidence has emerged indicating that the growth of a vast majority of tumors including gliomas is sustained by a subpopulation of cancer cells with stem cell properties called cancer initiating cells. These cells are able to initiate and propagate tumors and constitute only a fraction of all tumor cells. In the present study, we showed that intracerebral injection of cultured glioma-initiating cells into nude mice produced fast growing tumors showing necrosis and gadolinium enhancement in MR images, whereas gliomas produced by injecting freshly purified glioma-initiating cells grew slowly and showed no necrosis and very little gadolinium enhancement. Using proton localized spectroscopy at 14.1 Tesla, decreasing trends of N-acetylaspartate, glutamate and glucose concentrations and an increasing trend of glycine concentration were observed near the injection site after injecting cultured glioma-initiating cells. In contrast to the spectra of tumors grown from fresh cells, those from cultured cells showed intense peaks of lipids, increased absolute concentrations of glycine and choline-containing compounds, and decreased concentrations of glutamine, taurine and total creatine, when compared with a contralateral non-tumor-bearing brain tissue. A decrease in concentrations of N-acetylaspartate and γ-aminobutyrate was found in both tumor phenotypes after solid tumor formation. Further investigation is needed to determine the cause of the dissimilarities between the tumors grown from cultured glioma-initiating cells and those from freshly purified glioma-initiating cells, both derived from human glioblastomas.
Keywords AnimalsCell Line, TumorGlioma/metabolism/pathologyHumansMagnetic Resonance Spectroscopy/methodsMetabolic Clearance RateMiceMice, NudeNeoplasm Proteins/metabolismProtons/diagnostic useTumor Markers, Biological/metabolism
PMID: 21796713
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Research group CIBM (863)
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MLYNÁRIK, Vladimír et al. In vivo metabolic profiling of glioma-initiating cells using proton magnetic resonance spectroscopy at 14.1 Tesla. In: NMR in biomedicine, 2012, vol. 25, n° 4, p. 506-13. doi: 10.1002/nbm.1763 https://archive-ouverte.unige.ch/unige:32999

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Deposited on : 2014-01-09

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