Feasibility of in vivo 15N MRS detection of hyperpolarized 15N labeled choline in rats

Cudalbu, Cristina; Comment, Arnaud; Kurdzesau, Fiodar; van Heeswijk, Ruud B; Uffmann, Kai; Jannin, Sami; Denisov, Vladimir; Kirik, Deniz; Gruetter, Rolf

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Title		Feasibility of in vivo 15N MRS detection of hyperpolarized 15N labeled choline in rats
Authors		Cudalbu, Cristina Comment, Arnaud Kurdzesau, Fiodar van Heeswijk, Ruud B Uffmann, Kai Jannin, Sami Denisov, Vladimir Kirik, Deniz Gruetter, Rolf show all authors [1 - 9]
Published in		Physical Chemistry Chemical Physics. 2010, vol. 12, no. 22, p. 5818-23
Abstract		The increase of total choline in tumors has become an important biomarker in cancer diagnosis. Choline and choline metabolites can be measured in vivo and in vitro using multinuclear MRS. Recent in vivo(13)C MRS studies using labeled substrates enhanced via dynamic nuclear polarization demonstrated the tremendous potential of hyperpolarization for real-time metabolic studies. The present study demonstrates the feasibility of detecting hyperpolarized (15)N labeled choline in vivo in a rat head at 9.4 T. We furthermore report the in vitro (172 +/- 16 s) and in vivo (126 +/- 15 s) longitudinal relaxation times. We conclude that with appropriate infusion protocols it is feasible to detect hyperpolarized (15)N labeled choline in live animals.
Keywords		Animals — Choline/chemistry — Isotope Labeling — Magnetic Resonance Imaging/methods — Male — Nitrogen Isotopes/chemistry — Rats — Rats, Sprague-Dawley
Identifiers		DOI: 10.1039/c002309b PMID: 20461252
Full text		Article (Published version) (1.3 MB) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de radiologie et informatique médicale
Research group		CIBM (863)
Citation (ISO format)		CUDALBU, Cristina et al. Feasibility of in vivo 15N MRS detection of hyperpolarized 15N labeled choline in rats. In: Physical Chemistry Chemical Physics, 2010, vol. 12, n° 22, p. 5818-23. https://archive-ouverte.unige.ch/unige:32978