UNIGE document Scientific Article
previous document  unige:32973  next document
add to browser collection

Deep thiopental anesthesia alters steady-state glucose homeostasis but not the neurochemical profile of rat cortex

Duarte, Joao M N
Mlynarik, Vladimir
Published in Journal of neuroscience research. 2010, vol. 88, no. 2, p. 413-9
Abstract Barbiturates are regularly used as an anesthetic for animal experimentation and clinical procedures and are frequently provided with solubilizing compounds, such as ethanol and propylene glycol, which have been reported to affect brain function and, in the case of (1)H NMR experiments, originate undesired resonances in spectra affecting the quantification. As an alternative, thiopental can be administrated without any solubilizing agents. The aim of the study was to investigate the effect of deep thiopental anesthesia on the neurochemical profile consisting of 19 metabolites and on glucose transport kinetics in vivo in rat cortex compared with alpha-chloralose using localized (1)H NMR spectroscopy. Thiopental was devoid of effects on the neurochemical profile, except for the elevated glucose at a given plasma glucose level resulting from thiopental-induced depression of glucose consumption at isoelectrical condition. Over the entire range of plasma glucose levels, steady-state glucose concentrations were increased on average by 48% +/- 8%, implying that an effect of deep thiopental anesthesia on the transport rate relative to cerebral glucose consumption ratio was increased by 47% +/- 8% compared with light alpha-chloralose-anesthetized rats. We conclude that the thiopental-induced isoelectrical condition in rat cortex significantly affected glucose contents by depressing brain metabolism, which remained substantial at isoelectricity.
Keywords Anesthetics, Intravenous/pharmacologyAnimalsBlood Glucose/drug effectsCerebral Cortex/drug effects/metabolismChloralose/pharmacologyGlucose/metabolismHomeostasis/drug effectsIsoelectric PointKineticsMagnetic Resonance SpectroscopyMaleProtonsRatsRats, Sprague-DawleyThiopental/pharmacology
PMID: 19746430
Full text
Article (Published version) (193 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Research group CIBM (863)
(ISO format)
LEI, Hongxia et al. Deep thiopental anesthesia alters steady-state glucose homeostasis but not the neurochemical profile of rat cortex. In: Journal of neuroscience research, 2010, vol. 88, n° 2, p. 413-9. doi: 10.1002/jnr.22212 https://archive-ouverte.unige.ch/unige:32973

432 hits

0 download


Deposited on : 2014-01-08

Export document
Format :
Citation style :