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Scientific article
English

Deep thiopental anesthesia alters steady-state glucose homeostasis but not the neurochemical profile of rat cortex

Contributors

Lei, Hongxia; Duarte, Joao M N; Mlynarik, Vladimir; Python, Agathe; Gruetter, Rolf

Published in

Journal of neuroscience research, vol. 88, no. 2, p. 413-419

Publication date

2010

Abstract

Barbiturates are regularly used as an anesthetic for animal experimentation and clinical procedures and are frequently provided with solubilizing compounds, such as ethanol and propylene glycol, which have been reported to affect brain function and, in the case of (1)H NMR experiments, originate undesired resonances in spectra affecting the quantification. As an alternative, thiopental can be administrated without any solubilizing agents. The aim of the study was to investigate the effect of deep thiopental anesthesia on the neurochemical profile consisting of 19 metabolites and on glucose transport kinetics in vivo in rat cortex compared with alpha-chloralose using localized (1)H NMR spectroscopy. Thiopental was devoid of effects on the neurochemical profile, except for the elevated glucose at a given plasma glucose level resulting from thiopental-induced depression of glucose consumption at isoelectrical condition. Over the entire range of plasma glucose levels, steady-state glucose concentrations were increased on average by 48% +/- 8%, implying that an effect of deep thiopental anesthesia on the transport rate relative to cerebral glucose consumption ratio was increased by 47% +/- 8% compared with light alpha-chloralose-anesthetized rats. We conclude that the thiopental-induced isoelectrical condition in rat cortex significantly affected glucose contents by depressing brain metabolism, which remained substantial at isoelectricity.

Keywords

  • Anesthetics, Intravenous/pharmacology
  • Animals
  • Blood Glucose/drug effects
  • Cerebral Cortex/drug effects/metabolism
  • Chloralose/pharmacology
  • Glucose/metabolism
  • Homeostasis/drug effects
  • Isoelectric Point
  • Kinetics
  • Magnetic Resonance Spectroscopy
  • Male
  • Protons
  • Rats
  • Rats, Sprague-Dawley
  • Thiopental/pharmacology

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

LEI, Hongxia et al. Deep thiopental anesthesia alters steady-state glucose homeostasis but not the neurochemical profile of rat cortex. In: Journal of neuroscience research, 2010, vol. 88, n° 2, p. 413–419. doi: 10.1002/jnr.22212

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0360-4012
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Technical informations

Creation

12/12/2013 20:34:00

First validation

12/12/2013 20:34:00

Update

14/03/2023 20:49:13

Status update

14/03/2023 20:49:13

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