en
Scientific article
English

Quantification of brain glycogen concentration and turnover through localized 13C NMR of both the C1 and C6 resonances

Published inNMR in biomedicine, vol. 23, no. 3, p. 270-276
Publication date2010
Abstract

We have recently shown that at isotopic steady state (13)C NMR can provide a direct measurement of glycogen concentration changes, but that the turnover of glycogen was not accessible with this protocol. The aim of the present study was to design, implement and apply a novel dual-tracer infusion protocol to simultaneously measure glycogen concentration and turnover. After reaching isotopic steady state for glycogen C1 using [1-(13)C] glucose administration, [1,6-(13)C(2)] glucose was infused such that isotopic steady state was maintained at the C1 position, but the C6 position reflected (13)C label incorporation. To overcome the large chemical shift displacement error between the C1 and C6 resonances of glycogen, we implemented 2D gradient based localization using the Fourier series window approach, in conjunction with time-domain analysis of the resulting FIDs using jMRUI. The glycogen concentration of 5.1 +/- 1.6 mM measured from the C1 position was in excellent agreement with concomitant biochemical determinations. Glycogen turnover measured from the rate of label incorporation into the C6 position of glycogen in the alpha-chloralose anesthetized rat was 0.7 micromol/g/h.

Keywords
  • Animals
  • Brain/metabolism
  • Carbon/metabolism
  • Carbon Isotopes
  • Computer Simulation
  • Fourier Analysis
  • Glucose/metabolism
  • Glycogen/metabolism
  • Magnetic Resonance Spectroscopy
  • Rats
  • Rats, Sprague-Dawley
  • Time Factors
Research group
Citation (ISO format)
VAN HEESWIJK, Ruud B et al. Quantification of brain glycogen concentration and turnover through localized 13C NMR of both the C1 and C6 resonances. In: NMR in biomedicine, 2010, vol. 23, n° 3, p. 270–276. doi: 10.1002/nbm.1460
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0952-3480
511views
0downloads

Technical informations

Creation12/12/2013 8:41:00 PM
First validation12/12/2013 8:41:00 PM
Update time03/14/2023 8:49:10 PM
Status update03/14/2023 8:49:10 PM
Last indexation01/16/2024 1:51:54 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack