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Direct in vivo measurement of glycine and the neurochemical profile in the rat medulla oblongata

Xin, Lijing
Gambarota, Giulio
Duarte, João M N
Mlynárik, Vladimír
Published in NMR in Biomedicine. 2010, vol. 23, no. 9, p. 1097-102
Abstract The medulla oblongata (MO) contains a high density of glycinergic synapses and a particularly high concentration of glycine. The aims of this study were to measure directly in vivo the neurochemical profile, including glycine, in MO using a spin-echo-based (1)H MRS sequence at TE = 2.8 ms and to compare it with three other brain regions (cortex, striatum and hippocampus) in the rat. Glycine was quantified in MO at TE = 2.8 ms with a Cramér-Rao lower bound (CRLB) of approximately 5%. As a result of the relatively low level of glycine in the other three regions, the measurement of glycine was performed at TE = 20 ms, which provides a favorable J-modulation of overlapping myo-inositol resonance. The other 14 metabolites composing the neurochemical profile were quantified in vivo in MO with CRLBs below 25%. Absolute concentrations of metabolites in MO, such as glutamate, glutamine, γ-aminobutyrate, taurine and glycine, were in the range of previous in vitro quantifications in tissue extracts. Compared with the other regions, MO had a three-fold higher glycine concentration, and was characterised by reduced (p < 0.001) concentrations of glutamate (-50 ± 4%), glutamine (-54 ± 3%) and taurine (-78 ± 3%). This study suggests that the functional specialisation of distinct brain regions is reflected in the neurochemical profile.
Keywords AnimalsCerebral Cortex/chemistry/metabolismCorpus Striatum/chemistry/metabolismGlycine/metabolismHippocampus/chemistry/metabolismHumansMagnetic Resonance Spectroscopy/methodsMedulla Oblongata/chemistry/metabolismRatsRats, Sprague-Dawley
PMID: 20963803
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XIN, Lijing et al. Direct in vivo measurement of glycine and the neurochemical profile in the rat medulla oblongata. In: NMR in Biomedicine, 2010, vol. 23, n° 9, p. 1097-102. doi: 10.1002/nbm.1537 https://archive-ouverte.unige.ch/unige:32967

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Deposited on : 2014-01-08

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