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Scientific article
English

Evolution of the neurochemical profile after transient focal cerebral ischemia in the mouse brain

Contributors

Lei, Hongxia; Berthet, Carole; Hirt, Lorenz; Gruetter, Rolf

Published in

Journal of cerebral blood flow and metabolism, vol. 29, no. 4, p. 811-819

Publication date

2009

Abstract

Evolution of the neurochemical profile consisting of 19 metabolites after 30 mins of middle cerebral artery occlusion was longitudinally assessed at 3, 8 and 24 h in 6 to 8 microL volumes in the striatum using localized 1H-magnetic resonance spectroscopy at 14.1 T. Profound changes were detected as early as 3 h after ischemia, which include elevated lactate levels in the presence of significant glucose concentrations, decreases in glutamate and a transient twofold glutamine increase, likely to be linked to the excitotoxic release of glutamate and conversion into glial glutamine. Interestingly, decreases in N-acetyl-aspartate (NAA), as well as in taurine, exceeded those in neuronal glutamate, suggesting that the putative neuronal marker NAA is rather a sensitive marker of neuronal viability. With further ischemia evolution, additional, more profound concentration decreases were detected, reflecting a disruption of cellular functions. We conclude that early changes in markers of energy metabolism, glutamate excitotoxicity and neuronal viability can be detected with high precision non-invasively in mice after stroke. Such investigations should lead to a better understanding and insight into the sequential early changes in the brain parenchyma after ischemia, which could be used for identifying new targets for neuroprotection.

Keywords

  • Animals
  • Aspartic Acid/analogs & derivatives/analysis
  • Cerebrovascular Circulation
  • Corpus Striatum/metabolism
  • Glucose/analysis
  • Glutamic Acid/analysis
  • Glutamine/analysis
  • Infarction, Middle Cerebral Artery
  • Ischemic Attack, Transient/metabolism
  • Kinetics
  • Lactic Acid/analysis
  • Magnetic Resonance Spectroscopy
  • Metabolism
  • Mice

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

LEI, Hongxia et al. Evolution of the neurochemical profile after transient focal cerebral ischemia in the mouse brain. In: Journal of cerebral blood flow and metabolism, 2009, vol. 29, n° 4, p. 811–819. doi: 10.1038/jcbfm.2009.8

Main files (1)

Article (Published version)

Identifiers

Journal ISSN

0271-678X
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Technical informations

Creation

13/12/2013 08:20:00

First validation

13/12/2013 08:20:00

Update

14/03/2023 20:49:08

Status update

14/03/2023 20:49:07

Last indexation

30/10/2024 15:43:50
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