UNIGE document Scientific Article
previous document  unige:32952  next document
add to browser collection

In vivo 1H NMR measurement of glycine in rat brain at 9.4 T at short echo time

Gambarota, Giulio
Xin, Lijing
Kohler, Ingrid
Mlynárik, Vladimír
Published in Magnetic Resonance in Medicine. 2008, vol. 60, no. 3, p. 727-731
Abstract Glycine is an amino acid present in mammalian brain, where it acts as an inhibitory and excitatory neurotransmitter. The two detectable protons of glycine give rise to a singlet at 3.55 ppm that overlaps with the more intense myo-inositol resonances, and its measurement has traditionally required specific editing efforts. The aim of the current study was to reduce the signal intensity of myo-inositol relative to that of glycine by exploiting the fast signal J-evolution of the myo-inositol spin system when using a single spin-echo localization method we recently introduced. Glycine was detected at TE = 20 ms with an average Cramér-Rao lower bound (CRLB) of 8.6% +/- 1.5% in rat brain (N = 5), at 9.4 T. The concentration of glycine was determined using LCModel analysis at 1.1 +/- 0.1 mM, in good agreement with biochemical measurements previously reported. We conclude that at high magnetic fields, glycine can be measured at a relatively short echo time (TE) without additional editing efforts.
Keywords AnimalsBrain/metabolismGlycine/metabolismMagnetic Resonance Spectroscopy/methodsMaleRatsRats, Sprague-Dawley
PMID: 18727088
Full text
Article (Published version) (290 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Research group CIBM (863)
(ISO format)
GAMBAROTA, Giulio et al. In vivo 1H NMR measurement of glycine in rat brain at 9.4 T at short echo time. In: Magnetic Resonance in Medicine, 2008, vol. 60, n° 3, p. 727-731. doi: 10.1002/mrm.21695 https://archive-ouverte.unige.ch/unige:32952

381 hits

0 download


Deposited on : 2014-01-08

Export document
Format :
Citation style :