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Title

In vivo 1H NMR measurement of glycine in rat brain at 9.4 T at short echo time

Authors
Gambarota, Giulio
Xin, Lijing
Kohler, Ingrid
Mlynárik, Vladimír
Published in Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine. 2008, vol. 60, no. 3, p. 727-31
Abstract Glycine is an amino acid present in mammalian brain, where it acts as an inhibitory and excitatory neurotransmitter. The two detectable protons of glycine give rise to a singlet at 3.55 ppm that overlaps with the more intense myo-inositol resonances, and its measurement has traditionally required specific editing efforts. The aim of the current study was to reduce the signal intensity of myo-inositol relative to that of glycine by exploiting the fast signal J-evolution of the myo-inositol spin system when using a single spin-echo localization method we recently introduced. Glycine was detected at TE = 20 ms with an average Cramér-Rao lower bound (CRLB) of 8.6% +/- 1.5% in rat brain (N = 5), at 9.4 T. The concentration of glycine was determined using LCModel analysis at 1.1 +/- 0.1 mM, in good agreement with biochemical measurements previously reported. We conclude that at high magnetic fields, glycine can be measured at a relatively short echo time (TE) without additional editing efforts.
Keywords AnimalsBrain/metabolismGlycine/metabolismMagnetic Resonance Spectroscopy/methodsMaleRatsRats, Sprague-Dawley
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PMID: 18727088
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Research group CIBM (863)
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GAMBAROTA, Giulio et al. In vivo 1H NMR measurement of glycine in rat brain at 9.4 T at short echo time. In: Magnetic resonance in medicine : official journal of the Society of Magnetic Resonance in Medicine / Society of Magnetic Resonance in Medicine, 2008, vol. 60, n° 3, p. 727-31. https://archive-ouverte.unige.ch/unige:32952

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Deposited on : 2014-01-08

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