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Scientific article
English

In vivo 1H NMR measurement of glycine in rat brain at 9.4 T at short echo time

Published inMagnetic resonance in medicine, vol. 60, no. 3, p. 727-731
Publication date2008
Abstract

Glycine is an amino acid present in mammalian brain, where it acts as an inhibitory and excitatory neurotransmitter. The two detectable protons of glycine give rise to a singlet at 3.55 ppm that overlaps with the more intense myo-inositol resonances, and its measurement has traditionally required specific editing efforts. The aim of the current study was to reduce the signal intensity of myo-inositol relative to that of glycine by exploiting the fast signal J-evolution of the myo-inositol spin system when using a single spin-echo localization method we recently introduced. Glycine was detected at TE = 20 ms with an average Cramér-Rao lower bound (CRLB) of 8.6% +/- 1.5% in rat brain (N = 5), at 9.4 T. The concentration of glycine was determined using LCModel analysis at 1.1 +/- 0.1 mM, in good agreement with biochemical measurements previously reported. We conclude that at high magnetic fields, glycine can be measured at a relatively short echo time (TE) without additional editing efforts.

Keywords
  • Animals
  • Brain/metabolism
  • Glycine/metabolism
  • Magnetic Resonance Spectroscopy/methods
  • Male
  • Rats
  • Rats, Sprague-Dawley
Research group
Citation (ISO format)
GAMBAROTA, Giulio et al. In vivo 1H NMR measurement of glycine in rat brain at 9.4 T at short echo time. In: Magnetic resonance in medicine, 2008, vol. 60, n° 3, p. 727–731. doi: 10.1002/mrm.21695
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ISSN of the journal0740-3194
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