Scientific article

In vivo 1H NMR measurement of glycine in rat brain at 9.4 T at short echo time

Published inMagnetic resonance in medicine, vol. 60, no. 3, p. 727-731
Publication date2008

Glycine is an amino acid present in mammalian brain, where it acts as an inhibitory and excitatory neurotransmitter. The two detectable protons of glycine give rise to a singlet at 3.55 ppm that overlaps with the more intense myo-inositol resonances, and its measurement has traditionally required specific editing efforts. The aim of the current study was to reduce the signal intensity of myo-inositol relative to that of glycine by exploiting the fast signal J-evolution of the myo-inositol spin system when using a single spin-echo localization method we recently introduced. Glycine was detected at TE = 20 ms with an average Cramér-Rao lower bound (CRLB) of 8.6% +/- 1.5% in rat brain (N = 5), at 9.4 T. The concentration of glycine was determined using LCModel analysis at 1.1 +/- 0.1 mM, in good agreement with biochemical measurements previously reported. We conclude that at high magnetic fields, glycine can be measured at a relatively short echo time (TE) without additional editing efforts.

  • Animals
  • Brain/metabolism
  • Glycine/metabolism
  • Magnetic Resonance Spectroscopy/methods
  • Male
  • Rats
  • Rats, Sprague-Dawley
Research group
Citation (ISO format)
GAMBAROTA, Giulio et al. In vivo 1H NMR measurement of glycine in rat brain at 9.4 T at short echo time. In: Magnetic resonance in medicine, 2008, vol. 60, n° 3, p. 727–731. doi: 10.1002/mrm.21695
Main files (1)
Article (Published version)
ISSN of the journal0740-3194

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