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Non-invasive quantification of brain glycogen absolute concentration

Morgenthaler, Florence D
van Heeswijk, Ruud B
Xin, Lijing
Frenkel, Hanne
Published in Journal of Neurochemistry. 2008, vol. 107, no. 5, p. 1414-23
Abstract The only currently available method to measure brain glycogen in vivo is 13C NMR spectroscopy. Incorporation of 13C-labeled glucose (Glc) is necessary to allow glycogen measurement, but might be affected by turnover changes. Our aim was to measure glycogen absolute concentration in the rat brain by eliminating label turnover as variable. The approach is based on establishing an increased, constant 13C isotopic enrichment (IE). 13C-Glc infusion is then performed at the IE of brain glycogen. As glycogen IE cannot be assessed in vivo, we validated that it can be inferred from that of N-acetyl-aspartate IE in vivo: After [1-13C]-Glc ingestion, glycogen IE was 2.2 +/- 0.1 fold that of N-acetyl-aspartate (n = 11, R(2) = 0.77). After subsequent Glc infusion, glycogen IE equaled brain Glc IE (n = 6, paired t-test, p = 0.37), implying isotopic steady-state achievement and complete turnover of the glycogen molecule. Glycogen concentration measured in vivo by 13C NMR (mean +/- SD: 5.8 +/- 0.7 micromol/g) was in excellent agreement with that in vitro (6.4 +/- 0.6 micromol/g, n = 5). When insulin was administered, the stability of glycogen concentration was analogous to previous biochemical measurements implying that glycogen turnover is activated by insulin. We conclude that the entire glycogen molecule is turned over and that insulin activates glycogen turnover.
Keywords AnimalsAspartic Acid/analogs & derivatives/metabolismBrain/metabolismBrain ChemistryGlycogen/metabolismMagnetic Resonance Imaging/methodsMagnetic Resonance Spectroscopy/methodsRatsRats, Sprague-Dawley
PMID: 19013831
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Research group CIBM (863)
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MORGENTHALER, Florence D et al. Non-invasive quantification of brain glycogen absolute concentration. In: Journal of Neurochemistry, 2008, vol. 107, n° 5, p. 1414-23. https://archive-ouverte.unige.ch/unige:32944

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Deposited on : 2014-01-08

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