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Title

Perinatal iron deficiency predisposes the developing rat hippocampus to greater injury from mild to moderate hypoxia-ischemia

Authors
Rao, Raghavendra
Tkac, Ivan
Townsend, Elise L
Ennis, Kathleen
Georgieff, Michael K
Published in Journal of Cerebral Blood Flow and Metabolism. 2007, vol. 27, no. 4, p. 729-40
Abstract The hippocampus is injured in both hypoxia-ischemia (HI) and perinatal iron deficiency that are co-morbidities in infants of diabetic mothers and intrauterine growth restricted infants. We hypothesized that preexisting perinatal iron deficiency predisposes the hippocampus to greater injury when exposed to a relatively mild HI injury. Iron-sufficient and iron-deficient rats (hematocrit 40% lower and brain iron concentration 55% lower) were subjected to unilateral HI injury of 15, 30, or 45 mins (n=12 to 13/HI duration) on postnatal day 14. Sixteen metabolite concentrations were measured from an 11 microL volume on the ipsilateral (HI) and contralateral (control) hippocampi 1 week later using in vivo 1H NMR spectroscopy. The concentrations of creatine, glutamate, myo-inositol, and N-acetylaspartate were lower on the control side in the iron-deficient group (P<0.02, each). Magnetic resonance imaging showed hippocampal injury in the majority of the iron-deficient rats (58% versus 11%, P<0.0001) with worsening severity with increasing durations of HI (P=0.0001). Glucose, glutamate, N-acetylaspartate, and taurine concentrations were decreased and glutamine, lactate and myo-inositol concentrations, and glutamine/glutamate ratio were increased on the HI side in the iron-deficient group (P<0.01, each), mainly in the 30 and 45 mins HI subgroups (P<0.02, each). These neurochemical changes likely reflect the histochemically detected neuronal injury and reactive astrocytosis in the iron-deficient group and suggest that perinatal iron deficiency predisposes the hippocampus to greater injury from exposure to a relatively mild HI insult.
Keywords AnimalsAnimals, Newborn/physiologyAstrocytes/pathologyBody Weight/physiologyBrain ChemistryElectromagnetic FieldsFemaleHippocampus/growth & development/pathologyHistocytochemistryHypoxia-Ischemia, Brain/pathologyIron/deficiency/metabolismMagnetic Resonance ImagingMagnetic Resonance SpectroscopyMaleNeurons/pathologyOrgan Size/physiologyRatsRats, Sprague-Dawley
Identifiers
PMID: 16868555
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Research group CIBM (863)
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RAO, Raghavendra et al. Perinatal iron deficiency predisposes the developing rat hippocampus to greater injury from mild to moderate hypoxia-ischemia. In: Journal of Cerebral Blood Flow and Metabolism, 2007, vol. 27, n° 4, p. 729-40. https://archive-ouverte.unige.ch/unige:32936

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Deposited on : 2014-01-08

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