UNIGE document Scientific Article
previous document  unige:32930  next document
add to browser collection
Title

Neurochemical changes in Huntington R6/2 mouse striatum detected by in vivo 1H NMR spectroscopy

Authors
Tkac, Ivan
Dubinsky, Janet M
Keene, C Dirk
Low, Walter C
Published in Journal of Neurochemistry. 2007, vol. 100, no. 5, p. 1397-406
Abstract The neurochemical profile of the striatum of R6/2 Huntington's disease mice was examined at different stages of pathogenesis using in vivo(1)H NMR spectroscopy at 9.4 T. Between 8 and 12 weeks, R6/2 mice exhibited distinct changes in a set of 17 quantifiable metabolites compared with littermate controls. Concentrations of creatine, glycerophosphorylcholine, glutamine and glutathione increased and N-acetylaspartate decreased at 8 weeks. By 12 weeks, concentrations of phosphocreatine, taurine, ascorbate, glutamate, and myo-inositol increased and phophorylethanolamine decreased. These metabolic changes probably reflected multiple processes, including compensatory processes to maintain homeostasis, active at different stages in the development of HD. The observed changes in concentrations suggested impairment of neurotransmission, neuronal integrity and energy demand, and increased membrane breakdown, gliosis, and osmotic and oxidative stress. Comparisons between metabolite concentrations from individual animals clearly distinguished HD transgenics from non-diseased littermates and identified possible markers of disease progression. Metabolic changes in R6/2 striata were distinctly different from those observed previously in the quinolinic acid and 3NP models of HD. Longitudinal monitoring of changes in these metabolites may provide quantifiable measures of disease progression and treatment effects in both mouse models of HD and patients.
Keywords AnimalsCorpus Striatum/metabolismHuntington Disease/genetics/metabolismMagnetic Resonance SpectroscopyMiceMice, Transgenic
Identifiers
PMID: 17217418
Full text
Article (Published version) (776 Kb) - public document Free access
Structures
Research group CIBM (863)
Citation
(ISO format)
TKAC, Ivan et al. Neurochemical changes in Huntington R6/2 mouse striatum detected by in vivo 1H NMR spectroscopy. In: Journal of Neurochemistry, 2007, vol. 100, n° 5, p. 1397-406. https://archive-ouverte.unige.ch/unige:32930

174 hits

94 downloads

Update

Deposited on : 2014-01-08

Export document
Format :
Citation style :