en
Scientific article
English

Proton-observed carbon-edited NMR spectroscopy in strongly coupled second-order spin systems

Published inMagnetic resonance in medicine, vol. 55, no. 2, p. 250-257
Publication date2006
Abstract

Proton-observed carbon-edited (POCE) NMR spectroscopy is commonly used to measure 13C labeling with higher sensitivity compared to direct 13C NMR spectroscopy, at the expense of spectral resolution. For weakly coupled first-order spin systems, the multiplet signal at a specific proton chemical shift in POCE spectra directly reflects 13C enrichment of the carbon attached to this proton. The present study demonstrates that this is not necessarily the case for strongly coupled second-order spin systems. In such cases NMR signals can be detected in the POCE spectra even at chemical shifts corresponding to protons bound to 12C. This effect is demonstrated theoretically with density matrix calculations and simulations, and experimentally with measured POCE spectra of [3-13C]glutamate.

Keywords
  • Carbon Isotopes
  • Computer Simulation
  • Glutamic Acid/chemistry
  • Magnetic Resonance Spectroscopy/methods
  • Protons
  • Sensitivity and Specificity
Research group
Citation (ISO format)
HENRY, Pierre-Gilles et al. Proton-observed carbon-edited NMR spectroscopy in strongly coupled second-order spin systems. In: Magnetic resonance in medicine, 2006, vol. 55, n° 2, p. 250–257. doi: 10.1002/mrm.20764
Main files (1)
Article (Published version)
accessLevelRestricted
Identifiers
ISSN of the journal0740-3194
492views
0downloads

Technical informations

Creation12/16/2013 8:22:00 AM
First validation12/16/2013 8:22:00 AM
Update time03/14/2023 8:48:46 PM
Status update03/14/2023 8:48:46 PM
Last indexation01/16/2024 8:46:20 AM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack