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Scientific article
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Effect of chronic hypoglycaemia on glucose concentration and glycogen content in rat brain: A localized 13C NMR study

Published inJournal of neurochemistry, vol. 99, no. 1, p. 260-268
Publication date2006
Abstract

While chronic hypoglycaemia has been reported to increase unidirectional glucose transport across the blood-brain barrier (BBB) and to increase GLUT1 expression at the endothelium, the effect on steady-state brain d-glucose and brain glycogen content is currently unknown. Brain glucose and glycogen concentrations were directly measured in vivo using localized 13C magnetic resonance spectroscopy (MRS) following 12-14 days of hypoglycaemia. Brain glucose content was significantly increased by 48%, which is consistent with an increase in the maximal glucose transport rate, Tmax, by 58% compared with the sham-treated animals. The localized 13C NMR measurements of brain glucose were directly validated by comparison with biochemically determined brain glucose content after rapid focused microwave fixation (1.4 s at 4 kW). Both in vivo MRS and biochemical measurements implied that brain glycogen content was not affected by chronic hypoglycaemia, consistent with brain glucose being a major factor controlling brain glycogen content. We conclude that the increased glucose transporter expression in chronic hypoglycaemia leads to increased brain glucose content at a given level of glycaemia. Such increased brain glucose concentrations can result in a lowered glycaemic threshold of counter-regulation observed in chronic hypoglycaemia.

Keywords
  • Animals
  • Blood Glucose/metabolism
  • Blood-Brain Barrier
  • Brain/metabolism
  • Disease Models, Animal
  • Glucose/metabolism
  • Hypoglycemia/metabolism
  • Magnetic Resonance Spectroscopy
  • Rats
  • Reference Values
Research group
Citation (ISO format)
LEI, Hongxia, GRUETTER, Rolf. Effect of chronic hypoglycaemia on glucose concentration and glycogen content in rat brain: A localized 13C NMR study. In: Journal of neurochemistry, 2006, vol. 99, n° 1, p. 260–268. doi: 10.1111/j.1471-4159.2006.04115.x
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Article (Published version)
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ISSN of the journal0022-3042
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