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Quantification of vitamin C in the rat brain in vivo using short echo-time 1H MRS.

Terpstra, Melissa
Tkác, Ivan
Rao, Raghavendra
Published in Magnetic Resonance in Medicine. 2006, vol. 55, no. 5, p. 979-83
Abstract Both ultrashort echo-time STEAM and MEGA-PRESS-edited spectroscopy were used to validate noninvasive quantification of vitamin C (ascorbate) in the developing rat brain, where changes in ascorbate concentration have been reported. Despite strong overlap with resonances from glutamine, glutamate, glutathione, and macromolecules, reliable quantification of ascorbate (Cramer-Rao lower bounds<0.2 micromol/g) by LCModel analysis of STEAM (TE=2 ms) spectra was possible at 9.4 T. Ascorbate concentrations quantified from the STEAM spectra were in very good agreement with concentrations calculated from fully resolved ascorbate resonances in MEGA-PRESS-edited spectra measured from identical volumes of interest. Ascorbate concentrations measured using STEAM decreased with increasing postnatal rat age, in agreement with published brain ascorbate concentrations measured in vitro using high-performance liquid chromatography (HPLC).
Keywords Aging/metabolismAnimalsAnimals, NewbornAscorbic Acid/analysisBrain/metabolismFeasibility StudiesMagnetic Resonance Spectroscopy/methodsProtons/diagnostic useRatsReproducibility of ResultsSensitivity and SpecificityTime Factors
PMID: 16586452
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TERPSTRA, Melissa et al. Quantification of vitamin C in the rat brain in vivo using short echo-time 1H MRS. In: Magnetic Resonance in Medicine, 2006, vol. 55, n° 5, p. 979-83. doi: 10.1002/mrm.20854 https://archive-ouverte.unige.ch/unige:32907

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Deposited on : 2014-01-08

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