Culture-based detection of methicillin-resistant Staphylococcus aureus by a network of European laboratories: an external quality assessment study

Gazin, M; Lee, A; Derde, L; Kazma, M; Lammens, C; Ieven, M; Bonten, M; Carmeli, Y; Harbarth, Stéphan Juergen; Brun-Buisson, C; Goossens, H; Malhotra-Kumar, S

doi:10.1007/s10096-011-1499-0

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Title		Culture-based detection of methicillin-resistant Staphylococcus aureus by a network of European laboratories: an external quality assessment study
Authors		Gazin, M Lee, A Derde, L Kazma, M Lammens, C Ieven, M Bonten, M Carmeli, Y Harbarth, Stéphan Juergen Brun-Buisson, C Goossens, H Malhotra-Kumar, S show all authors [1 - 12]
Published in		European Journal of Clinical Microbiology & Infectious Diseases. 2012, vol. 31, no. 8, p. 1765-70
Abstract		Twenty-three hospital laboratories from Europe and Israel participated in an external quality assessment (EQA) of the culture-based detection of methicillin-resistant Staphylococcus aureus (MRSA). Participants also reported the MRSA prevalence in clinical cultures and patient screening specimens, as well as the MRSA screening practices employed at their hospitals. An EQA panel of 18 samples consisting of two MRSA harbouring SCCmec IV and I, and one strain each of methicillin-resistant coagulase-negative S. epidermidis, methicillin-sensitive S. aureus and Escherichia coli as pure strains or in mixtures at 10(7)-1 cfu absolute loads was analysed by the 23 participants. Seventeen (74%) participants identified 17 or more samples correctly. Of these, 15 (88%) utilised a chromogenic medium alone (ChromID, bioMérieux; BBL CHROMagar, BD Diagnostics; MRSA Select, Bio-Rad Laboratories) or combined with a conventional medium and up to three confirmatory tests. Proportions of MRSA among S. aureus isolated from clinical cultures varied widely, even among hospitals within countries, ranging from 11-20% to 61-70%. MRSA carriage rates were less variable (0-20%) between countries. Almost all participants (n=22, 96%) screened patients for MRSA carriage during 2009-2010, of which 15 (68%) screened intensive care unit (ICU) patients alone or combined with other targeted high-risk groups, and 10 (45%) combined nasal screening with another body site.
Keywords		Carrier State/diagnosis/microbiology — Chromogenic Compounds/diagnostic use — Culture Media/chemistry — Escherichia coli/drug effects/isolation & purification — Europe — Humans — International Cooperation — Israel — Laboratory Proficiency Testing — Methicillin-Resistant Staphylococcus aureus/growth & development/isolation & purification — Microbial Sensitivity Tests/methods/standards — Quality Assurance, Health Care — Staphylococcal Infections/diagnosis/microbiology — Staphylococcus epidermidis/drug effects/isolation & purification
Identifiers		DOI: 10.1007/s10096-011-1499-0 PMID: 22160823
Full text		Article (Published version) (139 Kb) - Limited access to UNIGE
Structures		Faculté de médecine / Section de médecine clinique / Département de médecine
Research group		Groupe Harbarth Stephan (Staphylocoques dorés résistants à la méthicilline) (866)
Citation (ISO format)		GAZIN, M et al. Culture-based detection of methicillin-resistant Staphylococcus aureus by a network of European laboratories: an external quality assessment study. In: European Journal of Clinical Microbiology & Infectious Diseases, 2012, vol. 31, n° 8, p. 1765-70. doi: 10.1007/s10096-011-1499-0 https://archive-ouverte.unige.ch/unige:32514