UNIGE document Scientific Article
previous document  unige:32514  next document
add to browser collection
Title

Culture-based detection of methicillin-resistant Staphylococcus aureus by a network of European laboratories: an external quality assessment study

Authors
Gazin, M
Lee, A
Derde, L
Kazma, M
Lammens, C
Ieven, M
Bonten, M
Carmeli, Y
show hidden authors show all authors [1 - 12]
Published in European Journal of Clinical Microbiology & Infectious Diseases. 2012, vol. 31, no. 8, p. 1765-70
Abstract Twenty-three hospital laboratories from Europe and Israel participated in an external quality assessment (EQA) of the culture-based detection of methicillin-resistant Staphylococcus aureus (MRSA). Participants also reported the MRSA prevalence in clinical cultures and patient screening specimens, as well as the MRSA screening practices employed at their hospitals. An EQA panel of 18 samples consisting of two MRSA harbouring SCCmec IV and I, and one strain each of methicillin-resistant coagulase-negative S. epidermidis, methicillin-sensitive S. aureus and Escherichia coli as pure strains or in mixtures at 10(7)-1 cfu absolute loads was analysed by the 23 participants. Seventeen (74%) participants identified 17 or more samples correctly. Of these, 15 (88%) utilised a chromogenic medium alone (ChromID, bioMérieux; BBL CHROMagar, BD Diagnostics; MRSA Select, Bio-Rad Laboratories) or combined with a conventional medium and up to three confirmatory tests. Proportions of MRSA among S. aureus isolated from clinical cultures varied widely, even among hospitals within countries, ranging from 11-20% to 61-70%. MRSA carriage rates were less variable (0-20%) between countries. Almost all participants (n=22, 96%) screened patients for MRSA carriage during 2009-2010, of which 15 (68%) screened intensive care unit (ICU) patients alone or combined with other targeted high-risk groups, and 10 (45%) combined nasal screening with another body site.
Keywords Carrier State/diagnosis/microbiologyChromogenic Compounds/diagnostic useCulture Media/chemistryEscherichia coli/drug effects/isolation & purificationEuropeHumansInternational CooperationIsraelLaboratory Proficiency TestingMethicillin-Resistant Staphylococcus aureus/growth & development/isolation & purificationMicrobial Sensitivity Tests/methods/standardsQuality Assurance, Health CareStaphylococcal Infections/diagnosis/microbiologyStaphylococcus epidermidis/drug effects/isolation & purification
Identifiers
PMID: 22160823
Full text
Article (Published version) (139 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Structures
Research group Groupe Harbarth Stephan (Staphylocoques dorés résistants à la méthicilline) (866)
Citation
(ISO format)
GAZIN, M et al. Culture-based detection of methicillin-resistant Staphylococcus aureus by a network of European laboratories: an external quality assessment study. In: European Journal of Clinical Microbiology & Infectious Diseases, 2012, vol. 31, n° 8, p. 1765-70. https://archive-ouverte.unige.ch/unige:32514

249 hits

0 download

Update

Deposited on : 2013-12-18

Export document
Format :
Citation style :