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MALAT-1, a non protein-coding RNA is upregulated in the cerebellum, hippocampus and brain stem of human alcoholics

Kryger, Rosemarie
Fan, Li
Wilce, Peter A
Published in Alcohol. 2012, vol. 46, no. 7, p. 629-34
Abstract Chronic alcohol intake induces neurochemical adaptative changes in the brain characterised by altered gene expression. A role for non-coding RNAs in alcoholism is beginning to emerge. PCR-differential display using total RNA extracted from brain material of human alcoholics and control cases identified a cDNA fragment corresponding to a section of a known non protein-coding RNA (ncRNA), MALAT-1, (also known as NEAT2). Comparison of mRNA levels of MALAT-1 was performed by northern and dot blot experiments using different regions of brain from human alcoholics and rats chronically treated with ethanol vapours and following withdrawal. A massive increase of MALAT-1 transcripts was detected in cerebellum of human alcoholics and increases were also noted in hippocampus and brain stem, while no significant increase of MALAT-1 expression was noted in frontal or motor cortices. In the rat no significant difference of MALAT-1 ortholog mRNA could be detected in cerebellum. In addition, similarly to humans, no significant increase of MALAT-1 expression was detected in cortex of alcohol-treated rats, however, after 24 h alcohol withdrawal, a significant upregulation of MALAT-1 expression was observed in rat cortex. MALAT-1 is upregulated in specific regions of the human alcoholic brain and following alcohol withdrawal in the rat. As MALAT-1 regulates RNA processing, this suggests that alcohol-induced upregulation of MALAT-1 represents an important novel mechanism for alcohol actions in the CNS.
Keywords AdultAgedAged, 80 and overAlcohol-Induced Disorders/geneticsAlcoholicsAlcoholism/geneticsAnimalsBlotting, NorthernBrain Stem/chemistry/drug effectsCase-Control StudiesCerebellum/chemistry/drug effectsDisease Models, AnimalEthanol/adverse effectsFemaleGene Expression Profiling/methodsHippocampus/chemistry/drug effectsHumansInhalation ExposureMaleMiddle AgedPolymerase Chain ReactionRNA, Long Untranslated/analysisRatsRats, WistarSubstance Withdrawal Syndrome/geneticsTime FactorsUp-Regulation
PMID: 22560368
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Research group Radicaux libres et cellules souches embryonnaires (60)
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KRYGER, Rosemarie et al. MALAT-1, a non protein-coding RNA is upregulated in the cerebellum, hippocampus and brain stem of human alcoholics. In: Alcohol, 2012, vol. 46, n° 7, p. 629-34. doi: 10.1016/j.alcohol.2012.04.002 https://archive-ouverte.unige.ch/unige:32455

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Deposited on : 2013-12-18

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