• Home
  1. Home
  2. Adipocyte dysfunction in a mouse model of polycystic ovary syndrome (PCOS): evidence of adipocyte hypertrophy and tissue-specific inflammation
Scientific article
OA Policy
English

Adipocyte dysfunction in a mouse model of polycystic ovary syndrome (PCOS): evidence of adipocyte hypertrophy and tissue-specific inflammation

Contributors

Marino, Joseph S; Iler, Jeffrey; Dowling, Abigail R; Chua, Streamson; Bruning, Jens C; Coppari, Roberto; Hill, Jennifer W

Published in

PloS one, vol. 7, no. 10, e48643

Publication date

2012

Abstract

Clinical research shows an association between polycystic ovary syndrome (PCOS) and chronic inflammation, a pathological state thought to contribute to insulin resistance. The underlying pathways, however, have not been defined. The purpose of this study was to characterize the inflammatory state of a novel mouse model of PCOS. Female mice lacking leptin and insulin receptors in pro-opiomelanocortin neurons (IR/LepR(POMC) mice) and littermate controls were evaluated for estrous cyclicity, ovarian and adipose tissue morphology, and body composition by QMR and CT scan. Tissue-specific macrophage infiltration and cytokine mRNA expression were measured, as well as circulating cytokine levels. Finally, glucose regulation during pregnancy was evaluated as a measure of risk for diabetes development. Forty-five percent of IR/LepR(POMC) mice showed reduced or absent ovulation. IR/LepR(POMC) mice also had increased fat mass and adipocyte hypertrophy. These traits accompanied elevations in macrophage accumulation and inflammatory cytokine production in perigonadal adipose tissue, liver, and ovary. These mice also exhibited gestational hyperglycemia as predicted. This report is the first to show the presence of inflammation in IR/LepR(POMC) mice, which develop a PCOS-like phenotype. Thus, IR/LepR(POMC) mice may serve as a new mouse model to clarify the involvement of adipose and liver tissue in the pathogenesis and etiology of PCOS, allowing more targeted research on the development of PCOS and potential therapeutic interventions.

Keywords

  • Adipocytes/metabolism/pathology
  • Adipose Tissue/metabolism/pathology
  • Animals
  • Blood Glucose/metabolism
  • Disease Models, Animal
  • Female
  • Gene Expression
  • Humans
  • Hypertrophy
  • Inflammation/genetics/metabolism
  • Interleukin-1beta/blood/genetics/metabolism
  • Interleukin-6/blood/genetics/metabolism
  • Liver/metabolism/pathology
  • Magnetic Resonance Imaging
  • Male
  • Mice
  • Mice, Knockout
  • Ovary/metabolism/pathology
  • Ovulation/genetics
  • Polycystic Ovary Syndrome/blood/genetics/metabolism
  • Pregnancy
  • Receptor, Insulin/genetics/metabolism
  • Receptors, Leptin/genetics/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tomography, X-Ray Computed

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

MARINO, Joseph S et al. Adipocyte dysfunction in a mouse model of polycystic ovary syndrome (PCOS): evidence of adipocyte hypertrophy and tissue-specific inflammation. In: PloS one, 2012, vol. 7, n° 10, p. e48643. doi: 10.1371/journal.pone.0048643

Main files (1)

Article (Published version)
CC BY

Identifiers

Journal ISSN

1932-6203
765views
449downloads

Technical informations

Creation

04/11/2013 10:36:00

First validation

04/11/2013 10:36:00

Update

14/03/2023 20:44:07

Status update

14/03/2023 20:44:06

Last indexation

29/09/2025 10:35:55
All rights reserved by Archive ouverte UNIGE and the University of Geneva
OpenAIRE Indexed & Validated