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Title

Extensive phytochemical investigation of the polar constituents of Diospyros bipindensis Gürke traditionally used by Baka pygmies

Authors
Cesari, Ilaria
Caccialanza, Gabriele
Moundipa, Paul F.
Brusotti, Gloria
Published in Phytochemistry. 2013, vol. 96, p. 279-287
Abstract The water maceration and methanolic extract of the stem barks of Diospyros bipindensis, which is a medicinal plant used in Cameroon by Baka pygmies, revealed a complex high-performance liquid chromatography (HPLC) profile primarily composed of coumarin and naphthoquinone glycosides. The methanolic and apolar extracts also exhibited significant antifungal activity on a TLC bioautography assay against Candida albicans. HPLC-microfractionation in 96-well plates combined with bioautography enabled the rapid localization of the antifungal compound that was identified by HPLC-PDA and HPLCMS analysis as plumbagin. These on-line structural information were also used to dereplicate four known compounds. The isolation of the polar constituents from the methanolic extract enabled the identification of eleven other compounds also present in the traditional preparation, nine of which are reported for the first time. The structures of those compounds were elucidated by UV, NMR and HR-MS analysis.
Keywords Diospyros bipindensisHigh-performance liquid chromatographyCandida albicansPlumbaginTraditional preparation
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Research group Phytochimie et produits naturels bioactifs
Projects FNS: Grant CR2313_143733
Italian Ministero dell’Università e della Ricerca Scientifica (grant no. 2009Z8YTYC)
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CESARI, Ilaria et al. Extensive phytochemical investigation of the polar constituents of Diospyros bipindensis Gürke traditionally used by Baka pygmies. In: Phytochemistry, 2013, vol. 96, p. 279-287. https://archive-ouverte.unige.ch/unige:31890

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Deposited on : 2013-12-09

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