Extensive phytochemical investigation of the polar constituents of Diospyros bipindensis Gürke traditionally used by Baka pygmies

Cesari, Ilaria; Ferreira Queiroz, Emerson; Favre-Godal, Quentin; Marcourt, Laurence; Caccialanza, Gabriele; Moundipa, Paul F.; Brusotti, Gloria; Wolfender, Jean-Luc

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Title		Extensive phytochemical investigation of the polar constituents of Diospyros bipindensis Gürke traditionally used by Baka pygmies
Authors		Cesari, Ilaria Ferreira Queiroz, Emerson Favre-Godal, Quentin Marcourt, Laurence Caccialanza, Gabriele Moundipa, Paul F. Brusotti, Gloria Wolfender, Jean-Luc
Published in		Phytochemistry. 2013, vol. 96, p. 279-287
Abstract		The water maceration and methanolic extract of the stem barks of Diospyros bipindensis, which is a medicinal plant used in Cameroon by Baka pygmies, revealed a complex high-performance liquid chromatography (HPLC) profile primarily composed of coumarin and naphthoquinone glycosides. The methanolic and apolar extracts also exhibited significant antifungal activity on a TLC bioautography assay against Candida albicans. HPLC-microfractionation in 96-well plates combined with bioautography enabled the rapid localization of the antifungal compound that was identified by HPLC-PDA and HPLCMS analysis as plumbagin. These on-line structural information were also used to dereplicate four known compounds. The isolation of the polar constituents from the methanolic extract enabled the identification of eleven other compounds also present in the traditional preparation, nine of which are reported for the first time. The structures of those compounds were elucidated by UV, NMR and HR-MS analysis.
Keywords		Diospyros bipindensis — High-performance liquid chromatography — Candida albicans — Plumbagin — Traditional preparation
Identifiers		DOI: 10.1016/j.phytochem.2013.09.005
Full text		Article (Published version) (1 MB) - Limited access to UNIGE Other version: http://linkinghub.elsevier.com/retrieve/pii/S0031942213003439
Structures		Faculté des sciences / Section des sciences pharmaceutiques
Research group		Phytochimie et produits naturels bioactifs
Projects		FNS: Grant CR2313_143733 Italian Ministero dell’Università e della Ricerca Scientifica (grant no. 2009Z8YTYC)
Citation (ISO format)		CESARI, Ilaria et al. Extensive phytochemical investigation of the polar constituents of Diospyros bipindensis Gürke traditionally used by Baka pygmies. In: Phytochemistry, 2013, vol. 96, p. 279-287. https://archive-ouverte.unige.ch/unige:31890