Direct determination of valproic acid in biological fluids by capillary electrophoresis with contactless conductivity detection

Belin-Kavran, Gamze; Krähenbühl, Stephan; Hauser, Peter C.

doi:10.1016/j.jchromb.2006.10.003

Scientific article

English

Direct determination of valproic acid in biological fluids by capillary electrophoresis with contactless conductivity detection

Contributors

Belin-Kavran, Gamze; Krähenbühl, Stephan; Hauser, Peter C.

Published in

Journal of chromatography. B, vol. 847, no. 2, p. 205-209

Publication date

2007

Abstract

Capacitively coupled contactless conductivity detection (C(4)D) is a new technique providing high sensitivity in capillary electrophoresis (CE) especially for small ions that can otherwise only be determined with indirect methods. In this work, direct determination and validation of valproic acid (VPA) in biological fluids was achieved using CE with C(4)D. VPA is of pharmacological interest because of its use in epilepsy and bipolar disorder. The running electrolyte solution used consisted of 10mM 2-(N-morpholino)ethane sulfonic acid (MES)/dl-histidine (His) and 50microM hexadecyltrimethylammonium bromide (HTAB) at pH 6.0. Caproic acid (CA) was selected as internal standard (IS). Analyses of VPA in serum, plasma and urine samples were performed in less than 3min. The interference of the sample matrix was reduced by deproteinization of the sample with acetonitrile (ACN). The effect of the solvent type and ratio on interference was investigated. The limits of detection (LOD) and quantitation (LOQ) of VPA in plasma samples were determined as 24 and 80ng/ml, respectively. The method is linear between the 2 and 150microg/ml, covering well the therapeutic range of VPA (50-100microg/ml).

Keywords

Electric Conductivity
Electrophoresis
Capillary
Humans
Molecular Structure
Reproducibility of Results

UNIGE affiliation entities

Faculté des sciences / Section de chimie et biochimie / Département de chimie physique

UNIGE research groups

Groupe Gülaçar

Citation (ISO format)

BELIN-KAVRAN, Gamze, KRÄHENBÜHL, Stephan, HAUSER, Peter C. Direct determination of valproic acid in biological fluids by capillary electrophoresis with contactless conductivity detection. In: Journal of chromatography. B, 2007, vol. 847, n° 2, p. 205–209. doi: 10.1016/j.jchromb.2006.10.003

Article (Published version)

Identifiers

PID : unige:3189
DOI : 10.1016/j.jchromb.2006.10.003
PMID : 17070114

Additional URL for this publication

http://www.sciencedirect.com/science/article/pii/S1570023206008294

Journal ISSN

1570-0232

710views

760downloads

Creation

21/09/2009 16:21:28

First validation

21/09/2009 16:21:28

Update

14/03/2023 15:13:47

Status update

14/03/2023 15:13:47

Last indexation

29/10/2024 12:12:00

Direct determination of valproic acid in biological fluids by capillary electrophoresis with contactless conductivity detection

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

Main files (1)

Identifiers

Additional URL for this publication

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation