UNIGE document Scientific Article
previous document  unige:3189  next document
add to browser collection
Title

Direct determination of valproic acid in biological fluids by capillary electrophoresis with contactless conductivity detection

Authors
Krähenbühl, Stephan
Hauser, Peter C.
Published in Journal of Chromatography. B. 2007, vol. 847, no. 2, p. 205-209
Abstract Capacitively coupled contactless conductivity detection (C(4)D) is a new technique providing high sensitivity in capillary electrophoresis (CE) especially for small ions that can otherwise only be determined with indirect methods. In this work, direct determination and validation of valproic acid (VPA) in biological fluids was achieved using CE with C(4)D. VPA is of pharmacological interest because of its use in epilepsy and bipolar disorder. The running electrolyte solution used consisted of 10mM 2-(N-morpholino)ethane sulfonic acid (MES)/dl-histidine (His) and 50microM hexadecyltrimethylammonium bromide (HTAB) at pH 6.0. Caproic acid (CA) was selected as internal standard (IS). Analyses of VPA in serum, plasma and urine samples were performed in less than 3min. The interference of the sample matrix was reduced by deproteinization of the sample with acetonitrile (ACN). The effect of the solvent type and ratio on interference was investigated. The limits of detection (LOD) and quantitation (LOQ) of VPA in plasma samples were determined as 24 and 80ng/ml, respectively. The method is linear between the 2 and 150microg/ml, covering well the therapeutic range of VPA (50-100microg/ml).
Keywords Electric ConductivityElectrophoresisCapillaryHumansMolecular StructureReproducibility of Results
Identifiers
PMID: 17070114
Full text
Structures
Research group Groupe Gülaçar
Citation
(ISO format)
BELIN-KAVRAN, Gamze, KRÄHENBÜHL, Stephan, HAUSER, Peter C. Direct determination of valproic acid in biological fluids by capillary electrophoresis with contactless conductivity detection. In: Journal of Chromatography. B, 2007, vol. 847, n° 2, p. 205-209. https://archive-ouverte.unige.ch/unige:3189

198 hits

320 downloads

Update

Deposited on : 2009-09-21

Export document
Format :
Citation style :