Advanced Methods for Natural Product Drug Discovery in the Field of Nutraceuticals

Wolfender, Jean-Luc; Eugster, Philippe; Bohni, Nadine; Cuendet, Muriel

doi:10.2533/chimia.2011.400

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Title		Advanced Methods for Natural Product Drug Discovery in the Field of Nutraceuticals
Authors		Wolfender, Jean-Luc Eugster, Philippe Bohni, Nadine Cuendet, Muriel
Published in		Chimia. 2011, vol. 65, no. 6, p. 400-406
Abstract		Advances in analytical methods and bioassay development have helped to push forward the research in natural products. In plant extracts and nutraceuticals, bioactive compounds are part of a complex mixture. The development of high-resolution methods related to HPLC for both chemical and biological profiling has significantly increased the efficiency of classical bioactivity-guided fractionation procedures. Furthermore, the level of sensitivity obtained by these methods give the possibility to work with few micrograms of compound. This represents a key advantage for rapid localisation of the biological activity and subsequent identification of the compounds of interest. The same methods are also used to study the extracts from a metabolomic view point. The possibility to study them as a whole can highlight synergetic effects, which are likely to occur in plant extracts and nutraceuticals. In this paper, the main trends are summarised and the developments made in our laboratory on profiling crude extracts with UHPLC-TOF-MS, natural product identification at the microgram level using microflow NMR and integration of these methods with biological evaluation are highlighted.
Keywords		Biological activity — Metabolomics — Microflow NMR — Nutraceuticals — UHPLC-TOF-MS
Identifiers		DOI: 10.2533/chimia.2011.400 PMID: 21797168
Full text		Article (Published version) (423 Kb) - Free access
Structures		Faculté des sciences / Section des sciences pharmaceutiques
Research group		Phytochimie et produits naturels bioactifs
Projects		FNS: 205320-124667/1 FNS: CRSII3_127187
Citation (ISO format)		WOLFENDER, Jean-Luc et al. Advanced Methods for Natural Product Drug Discovery in the Field of Nutraceuticals. In: Chimia, 2011, vol. 65, n° 6, p. 400-406. doi: 10.2533/chimia.2011.400 https://archive-ouverte.unige.ch/unige:31160