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Titles listHepatocyte growth factor inhibits CNS autoimmunity by inducing tolerogenic dendritic cells and CD25+Foxp3+...
Titles listHepatocyte growth factor inhibits CNS autoimmunity by inducing tolerogenic dendritic cells and CD25+Foxp3+...
Scientific Article
unige:31021 
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Hepatocyte growth factor inhibits CNS autoimmunity by inducing tolerogenic dendritic cells and CD25+Foxp3+ regulatory T cells |
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| Published in | Proceedings of the National Academy of Sciences. 2010, vol. 107, no. 14, p. 6424-9 | |
| Abstract | Immune-mediated diseases of the CNS, such as multiple sclerosis and its animal model, experimental autoimmune encephalitis (EAE), are characterized by the activation of antigen-presenting cells and the infiltration of autoreactive lymphocytes within the CNS, leading to demyelination, axonal damage, and neurological deficits. Hepatocyte growth factor (HGF) is a pleiotropic factor known for both neuronal and oligodendrocytic protective properties. Here, we assess the effect of a selective overexpression of HGF by neurons in the CNS of C57BL/6 mice carrying an HGF transgene (HGF-Tg mice). EAE induced either by immunization with myelin oligodendrocyte glycoprotein peptide or by adoptive transfer of T cells was inhibited in HGF-Tg mice. Notably, the level of inflammatory cells infiltrating the CNS decreased, except for CD25(+)Foxp3(+) regulatory T (T(reg)) cells, which increased. A strong T-helper cell type 2 cytokine bias was observed: IFN-gamma and IL-12p70 decreased in the spinal cord of HGF-Tg mice, whereas IL-4 and IL-10 increased. Antigen-specific response assays showed that HGF is a potent immunomodulatory factor that inhibits dendritic cell (DC) function along with differentiation of IL-10-producing T(reg) cells, a decrease in IL-17-producing T cells, and down-regulation of surface markers of T-cell activation. These effects were reversed fully when DC were pretreated with anti-cMet (HGF receptor) antibodies. Our results suggest that, by combining both potentially neuroprotective and immunomodulatory effects, HGF is a promising candidate for the development of new treatments for immune-mediated demyelinating diseases associated with neurodegeneration such as multiple sclerosis. | |
| Keywords | Animals — Antigen Presentation — Cell Proliferation — Cytokines/biosynthesis/immunology — Dendritic Cells/immunology — Encephalomyelitis, Autoimmune, Experimental/genetics/immunology — Forkhead Transcription Factors/immunology — Hepatocyte Growth Factor/genetics/immunology — Immune Tolerance — Interleukin-2 Receptor alpha Subunit/immunology — Lymphocyte Activation — Mice — Mice, Inbred C57BL — Mice, Transgenic — Spleen/cytology/immunology — T-Lymphocytes, Regulatory/cytology/immunology — Th2 Cells/immunology | |
| Identifiers | PMID: 20332205 | |
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| Research group | Scléroses en plaques, neuroimmunologie, encéphalite autoimmune expérimentale (843) | |
| Citation (ISO format) | BENKHOUCHA, Mahdia et al. Hepatocyte growth factor inhibits CNS autoimmunity by inducing tolerogenic dendritic cells and CD25+Foxp3+ regulatory T cells. In: Proceedings of the National Academy of Sciences, 2010, vol. 107, n° 14, p. 6424-9. doi: 10.1073/pnas.0912437107 https://archive-ouverte.unige.ch/unige:31021 |
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