Scientific article

Secretory lysosomes in Dictyostelium: visualization, characterization, and dynamics

Published inMethods in molecular biology, vol. 983, p. 445-459
Publication date2013

Dictyostelium discoideum is a unicellular eukaryotic model largely used for the study of the endocytic pathway, mainly due to its high resemblance to the mammalian pathway. Over the last years, Dictyostelium has been increasingly employed for investigating the biogenesis and secretion of secretory lysosomes, given its advantage over mammalian cells. Here we describe protocols for determination of ionic concentration and rates of maturation and exocytosis of these compartments.

  • Calcium/metabolism
  • Dictyostelium/metabolism/ultrastructure
  • Endosomes/metabolism/ultrastructure
  • Exocytosis
  • Fluorescent Antibody Technique, Indirect
  • Hydrogen-Ion Concentration
  • Lysosomes/metabolism/secretion/ultrastructure
  • Protozoan Proteins/metabolism
  • Secretory Pathway
  • Secretory Vesicles
  • Single-Cell Analysis/methods
Research group
Citation (ISO format)
LIMA, Wanessa Cristina, COSSON, Pierre. Secretory lysosomes in Dictyostelium: visualization, characterization, and dynamics. In: Methods in molecular biology, 2013, vol. 983, p. 445–459. doi: 10.1007/978-1-62703-302-2_25
ISSN of the journal1064-3745

Technical informations

Creation10/07/2013 8:57:00 PM
First validation10/07/2013 8:57:00 PM
Update time03/14/2023 8:35:27 PM
Status update03/14/2023 8:35:27 PM
Last indexation10/18/2023 4:59:38 PM
All rights reserved by Archive ouverte UNIGE and the University of GenevaunigeBlack