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Imaging of synaptic proteins dynamics in vivo

Defense Thèse de doctorat : Univ. Genève et Lausanne, 2013 - Neur. 112 - 2013/09/24
Abstract In the adult somatosensory cortex the majority of the spines are stable. However some spines appear and disappear over time. EM analysis showed that many newly formed spines do not bear a morphologically defined synapse. In this thesis I describe studies that we have conducted to directly probe the dynamics of excitatory and inhibitory synapses in vivo. We used in utero electroporation of GFP-tagged PSD-95 to investigate the relationship between spine and PSD stability in neocortical L2/3 pyramidal cells in vivo. We then adapted a single cell electroporation method for chronic imaging of synaptic proteins. The technique has a high spatial and cell type selectivity, a low background fluorescence and does not affect developmental processes. As a proof of principle we transfected various synaptic proteins, some of which where thus far only successfully studied in vitro.
Keywords Synaptic plasticityIn vivoTwo photon.
URN: urn:nbn:ch:unige-308144
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CANE, Michele. Imaging of synaptic proteins dynamics in vivo. Université de Genève. Thèse, 2013. https://archive-ouverte.unige.ch/unige:30814

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Deposited on : 2013-10-30

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