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The TAO kinase KIN-18 regulates contractility and establishment of polarity in the C. elegans embryo

Spiga, Fabio M
Published in Developmental biology. 2013, vol. 373, no. 1, p. 26-38
Abstract Cell polarity is crucial for many aspects of cell and developmental biology. Cytoskeleton remodeling plays an essential role in the establishment of cell polarity. In the Caenorhabditis elegans one-cell embryo, while the actomyosin cytoskeleton is required for asymmetric localization of the PAR proteins, anterior PAR proteins exert a feedback regulation on contractility. Here we identify the TAO kinase KIN-18 as a regulator of cortical contractility in the early embryo. KIN-18 negatively regulates cortical contractions in a RHO-1 dependent manner and regulates RHO-1 cortical localization. KIN-18 contributes to polarity establishment by regulating the position of the boundary between anterior and posterior PAR proteins. Although KIN-18 is involved in polarity establishment, depletion of KIN-18 restores contractions in a par-3 mutant indicating that kin-18 is epistatic to par-3. We suggest a model in which KIN-18 provides a link between the cytoskeleton remodeling and polarity machineries, uncovering a role for TAO kinases in the regulation of cell polarity.
Keywords AnimalsCaenorhabditis elegans/embryology/geneticsCaenorhabditis elegans Proteins/genetics/metabolism/physiologyCell Cycle/genetics/physiologyCell Polarity/genetics/physiologyCytoskeleton/genetics/physiologyEpistasisGenetic/geneticsFluorescent Antibody TechniqueImage ProcessingComputer-AssistedMicroscopyInterferenceProtein Kinases/metabolism/physiologyRNA InterferenceTwo-Hybrid System TechniquesRho GTP-Binding Proteins/metabolism
PMID: 23064028
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Research group La division asymétrique (847)
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SPIGA, Fabio M, PROUTEAU, Manoel, GOTTA, Monica. The TAO kinase KIN-18 regulates contractility and establishment of polarity in the C. elegans embryo. In: Developmental biology, 2013, vol. 373, n° 1, p. 26-38. doi: 10.1016/j.ydbio.2012.10.001 https://archive-ouverte.unige.ch/unige:30784

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Deposited on : 2013-10-29

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