Organization of polar groups of 9 kd calbindin around Ca2+ ions bound to the protein: a microdielectric study

Wesolowski, Tomasz Adam; Boguta, Grzegorz; Bierzynski, Andrzej

doi:10.1093/protein/4.2.121

Scientific article

English

Organization of polar groups of 9 kd calbindin around Ca2+ ions bound to the protein: a microdielectric study

Contributors

Wesolowski, Tomasz Adam; Boguta, Grzegorz; Bierzynski, Andrzej

Published in

Protein engineering, design & selection, vol. 4, no. 4, p. 121-124

Publication date

1990

Abstract

Using a simple model of linear response of polarizable centers to an electric field, function {delta} is defined to characterize structural organization of protein polar groups. The function makes it possible to detect specific structures of nonuniformly distributed and mutually coupled groups that can transmit local structural and charge density perturbations, induced by an ion over long distances to functionally active sites of a protein molecule. In 9 kd calbindin (a small protein from the troponin C superfamily) two structural chains have been demonstrated that link together both Ca2+ ions coordinated by the protein. The chains form a rigid structure stabilized by coordination of one of the ions, so that binding of the other is promoted. Such a structure is probably common to all members of the superfamily and plays an important role in the mechanism of calcium binding by these proteins.

Keywords

9 kd calbindin
Calcium binding
Function δ
Protein polar groups
Troponin C superfamily

UNIGE affiliation entities

Faculté des sciences / Section de chimie et biochimie / Département de chimie physique

UNIGE research groups

Groupe Wesolowski

Citation (ISO format)

WESOLOWSKI, Tomasz Adam, BOGUTA, Grzegorz, BIERZYNSKI, Andrzej. Organization of polar groups of 9 kd calbindin around Ca2+ ions bound to the protein: a microdielectric study. In: Protein engineering, design & selection, 1990, vol. 4, n° 4, p. 121–124. doi: 10.1093/protein/4.2.121

Identifiers

PID : unige:3059
DOI : 10.1093/protein/4.2.121

Journal ISSN

1741-0126

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21/09/2009 16:19:50

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21/09/2009 16:19:50

Update

14/03/2023 15:13:22

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14/03/2023 15:13:22

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29/10/2024 12:08:51

Organization of polar groups of 9 kd calbindin around Ca2+ ions bound to the protein: a microdielectric study

Contributors

Published in

Publication date

Abstract

Keywords

UNIGE affiliation entities

UNIGE research groups

Citation (ISO format)

Identifiers

Journal ISSN

Technical informations

Creation

First validation

Update

Status update

Last indexation