Scientific Article
previous document  unige:30562  next document
add to browser collection
Title

The genome sequence of the malaria mosquito Anopheles gambiae

Authors
Holt, Robert A
Subramanian, G Mani
Halpern, Aaron
Sutton, Granger G
Charlab, Rosane
Nusskern, Deborah R
Wincker, Patrick
Clark, Andrew G
show hidden authors show all authors [1 - 123]
Published in Science. 2002, vol. 298, no. 5591, p. 129-49
Abstract Anopheles gambiae is the principal vector of malaria, a disease that afflicts more than 500 million people and causes more than 1 million deaths each year. Tenfold shotgun sequence coverage was obtained from the PEST strain of A. gambiae and assembled into scaffolds that span 278 million base pairs. A total of 91% of the genome was organized in 303 scaffolds; the largest scaffold was 23.1 million base pairs. There was substantial genetic variation within this strain, and the apparent existence of two haplotypes of approximately equal frequency ("dual haplotypes") in a substantial fraction of the genome likely reflects the outbred nature of the PEST strain. The sequence produced a conservative inference of more than 400,000 single-nucleotide polymorphisms that showed a markedly bimodal density distribution. Analysis of the genome sequence revealed strong evidence for about 14,000 protein-encoding transcripts. Prominent expansions in specific families of proteins likely involved in cell adhesion and immunity were noted. An expressed sequence tag analysis of genes regulated by blood feeding provided insights into the physiological adaptations of a hematophagous insect.
Keywords AnimalsAnopheles/classification/genetics/parasitology/physiologyBiological EvolutionBloodChromosome InversionChromosomes, Artificial, BacterialComputational BiologyDNA Transposable ElementsDigestionDrosophila melanogaster/geneticsEnzymes/chemistry/genetics/metabolismExpressed Sequence TagsFeeding BehaviorGene Expression RegulationGenes, InsectGenetic VariationGenomeHaplotypesHumansInsect Proteins/chemistry/genetics/physiologyInsect Vectors/genetics/parasitology/physiologyMalaria, Falciparum/transmissionMolecular Sequence DataMosquito ControlPhysical Chromosome MappingPlasmodium falciparum/growth & developmentPolymorphism, Single NucleotideProteomeSequence Analysis, DNASpecies SpecificityTranscription Factors/chemistry/genetics/physiology
Identifiers
PMID: 12364791
Full text
Article (Published version) (709 Kb) - document accessible for UNIGE members only Limited access to UNIGE
Citation
(ISO format)
HOLT, Robert A et al. The genome sequence of the malaria mosquito Anopheles gambiae. In: Science, 2002, vol. 298, n° 5591, p. 129-49. https://archive-ouverte.unige.ch/unige:30562

191 hits

1 download

Update

Deposited on : 2013-10-22

Export document
Format :
Citation style :