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Membrane shape at the edge of the dynamin helix sets location and duration of the fission reaction

Klein, Marius
Manzi, John
Dinis, Luis
Lenz, Martin
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Published in Cell. 2012, vol. 151, no. 3, p. 619-629
Abstract The GTPase dynamin polymerizes into a helical coat that constricts membrane necks of endocytic pits to promote their fission. However, the dynamin mechanism is still debated because constriction is necessary but not sufficient for fission. Here, we show that fission occurs at the interface between the dynamin coat and the uncoated membrane. At this location, the considerable change in membrane curvature increases the local membrane elastic energy, reducing the energy barrier for fission. Fission kinetics depends on tension, bending rigidity, and the dynamin constriction torque. Indeed, we experimentally find that the fission rate depends on membrane tension in vitro and during endocytosis in vivo. By estimating the energy barrier from the increased elastic energy at the edge of dynamin and measuring the dynamin torque, we show that the mechanical energy spent on dynamin constriction can reduce the energy barrier for fission sufficiently to promote spontaneous fission. :
Keywords AnimalsCOS CellsCell Membrane/metabolismCercopithecus aethiopsDynamins/metabolismEndocytosisGuanosine Triphosphate/metabolismModels, BiologicalSNARE Proteins/metabolism
PMID: 23101629
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Swiss National Science Foundation: 131003A_130520
Autre: HFSP Career Development Award 0061/2008
(ISO format)
MORLOT, Sandrine et al. Membrane shape at the edge of the dynamin helix sets location and duration of the fission reaction. In: Cell, 2012, vol. 151, n° 3, p. 619-629. doi: 10.1016/j.cell.2012.09.017 https://archive-ouverte.unige.ch/unige:30275

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Deposited on : 2013-10-07

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